recent development of human three-dimensional organoid cultures has opened new doors and opportunities ranging from modelling human development in vitro to personalised cancer therapies. these new in vitro systems are opening new horizons to the classic understanding of human development and disease. however, the complexity and heterogeneity of these models requires cutting-edge techniques to capture and trace global changes in gene expression to enable identification of key players and uncover the underlying molecular mechanisms. rapid development of sequencing approaches made possible global transcriptome analyses and epigenetic profiling. despite challenges in organoid culture and handling, these techniques are now being adapted to embrace organoids derived from a wide range of human tissues. here, we review current state-of-the-art multi-omics technologies, such as single-cell transcriptomics and chromatin accessibility assays, employed to study organoids as a model for development and a platform for precision medicine.

Smirnov, A., Melino, G., Candi, E. (2023). Gene expression in organoids: an expanding horizon. BIOLOGY DIRECT, 18(1) [10.1186/s13062-023-00360-2].

Gene expression in organoids: an expanding horizon

Smirnov A.;Melino G.;Candi E.
2023-01-01

Abstract

recent development of human three-dimensional organoid cultures has opened new doors and opportunities ranging from modelling human development in vitro to personalised cancer therapies. these new in vitro systems are opening new horizons to the classic understanding of human development and disease. however, the complexity and heterogeneity of these models requires cutting-edge techniques to capture and trace global changes in gene expression to enable identification of key players and uncover the underlying molecular mechanisms. rapid development of sequencing approaches made possible global transcriptome analyses and epigenetic profiling. despite challenges in organoid culture and handling, these techniques are now being adapted to embrace organoids derived from a wide range of human tissues. here, we review current state-of-the-art multi-omics technologies, such as single-cell transcriptomics and chromatin accessibility assays, employed to study organoids as a model for development and a platform for precision medicine.
2023
Pubblicato
Rilevanza internazionale
Review
Esperti anonimi
Settore BIO/10
English
Epigenetics
Omics
Organoids
Single-cell
Transcriptomics
Smirnov, A., Melino, G., Candi, E. (2023). Gene expression in organoids: an expanding horizon. BIOLOGY DIRECT, 18(1) [10.1186/s13062-023-00360-2].
Smirnov, A; Melino, G; Candi, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/363823
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