Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.

Koopmann, R., Cupelli, K., Redecke, L., Nass, K., Deponte, D.p., White, T.a., et al. (2012). In vivo protein crystallization opens new routes in structural biology. NATURE METHODS, 9(3), 259-262 [10.1038/nmeth.1859].

In vivo protein crystallization opens new routes in structural biology

Stellato F.;
2012-01-01

Abstract

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
2012
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore FIS/07
English
Con Impact Factor ISI
Koopmann, R., Cupelli, K., Redecke, L., Nass, K., Deponte, D.p., White, T.a., et al. (2012). In vivo protein crystallization opens new routes in structural biology. NATURE METHODS, 9(3), 259-262 [10.1038/nmeth.1859].
Koopmann, R; Cupelli, K; Redecke, L; Nass, K; Deponte, Dp; White, Ta; Stellato, F; Rehders, D; Liang, M; Andreasson, J; Aquila, A; Bajt, S; Barthelmess, M; Barty, A; Bogan, Mj; Bostedt, C; Boutet, S; Bozek, Jd; Caleman, C; Coppola, N; Davidsson, J; Doak, Rb; Ekeberg, T; Epp, Sw; Erk, B; Fleckenstein, H; Foucar, L; Graafsma, H; Gumprecht, L; Hajdu, J; Hampton, Cy; Hartmann, A; Hartmann, R; Hauser, G; Hirsemann, H; Holl, P; Hunter, Ms; Kassemeyer, S; Kirian, Ra; Lomb, L; Maia, Frnc; Kimmel, N; Martin, Av; Messerschmidt, M; Reich, C; Rolles, D; Rudek, B; Rudenko, A; Schlichting, I; Schulz, J; Seibert, Mm; Shoeman, Rl; Sierra, Rg; Soltau, H; Stern, S; Strulder, L; Timneanu, N; Ullrich, J; Wang, X; Weidenspointner, G; Weierstall, U; Williams, Gj; Wunderer, Cb; Fromme, P; Spence, Jch; Stehle, T; Chapman, Hn; Betzel, C; Duszenko, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/354247
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