Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Koopmann, R., Cupelli, K., Redecke, L., Nass, K., Deponte, D.p., White, T.a., et al. (2012). In vivo protein crystallization opens new routes in structural biology. NATURE METHODS, 9(3), 259-262 [10.1038/nmeth.1859].
In vivo protein crystallization opens new routes in structural biology
Stellato F.;
2012-01-01
Abstract
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.| File | Dimensione | Formato | |
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