How is the yeast proteome wired? This important question, central in yeast systems biology, remains unanswered in spite of the abundance of protein interaction data from high-throughput experiments. Unfortunately, these large-scale studies show striking discrepancies in their results and coverage such that biologists scrutinizing the "interactome" are often confounded by a mix of established physical interactions, functional associations, and experimental artifacts. This stimulated early attempts to integrate the available information and produce a list of protein interactions ranked according to an estimated functional reliability. The recent publication of the results of two large protein interaction experiments and the completion of a comprehensive literature curation effort has more than doubled the available information on the wiring of the yeast proteome. This motivates a fresh approach to the compilation of a yeast interactome based purely on evidence of physical interaction. We present a procedure exploiting both heuristic and probabilistic strategies to draft the yeast interactome taking advantage of various heterogeneous data sources: application of tandem affinity purification coupled to MS (TAP-MS), large-scale yeast two-hybrid studies, and results of small-scale experiments stored in dedicated databases. The end result is WI-PHL a weighted network encompassing a large majority of yeast proteins.
Kiemer, L., Costa S , Ueffing, M., & Cesareni, G. (2007). WI-PHI: A weighted yeast interactome enriched for direct physical interactions. PROTEOMICS, 7(6), 932-943.
|Tipologia:||Articolo su rivista|
|Citazione:||Kiemer, L., Costa S , Ueffing, M., & Cesareni, G. (2007). WI-PHI: A weighted yeast interactome enriched for direct physical interactions. PROTEOMICS, 7(6), 932-943.|
|Settore Scientifico Disciplinare:||Settore BIO/18 - Genetica|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/pmic.200600448|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2007|
|Titolo:||WI-PHI: A weighted yeast interactome enriched for direct physical interactions|
|Autori:||Kiemer, L ; Costa S ; Ueffing, M ; Cesareni, G|
|Appare nelle tipologie:||01 - Articolo su rivista|