p63 is a developmentally regulated transcription factor related to p53. It is involved in the development of ectodermal tissues, including limb, skin and in general, multilayered epithelia. The DeltaNp63alpha isoform is thought to play a 'master' role in the asymmetric division of epithelial cells. It is also involved in the pathogenesis of several human diseases, phenotypically characterized by ectodermal dysplasia. Our understanding of transcriptional networks controlled by p63 is limited, owing to the low number of bona fide targets. To screen for new targets, we employed chromatin immunoprecipitation from keratinocytes (KCs) coupled to the microarray technology, using both CpG islands and promoter arrays. The former revealed 96 loci, the latter yielded 85 additional genes. We tested 40 of these targets in several functional assays, including: (i) in vivo binding by p63 in primary KCs; (ii) expression analysis in differentiating HaCaT cells and in cells overexpressing DeltaNp63alpha; (iii) promoter transactivation and (iv) immunostaining in normal tissues, confirming their regulation by p63. We discovered several new specific targets whose functional categorization links p63 to cell growth and differentiation.

Vigano, M.A., Lamartine, J., Testoni, B., Merico, D., Alotto, D., Castagnoli, C., et al. (2006). New p63 targets in keratinocytes identified by a genome-wide approach. EMBO JOURNAL, 25(21), 5105-5116 [10.1038/sj.emboj.7601375].

New p63 targets in keratinocytes identified by a genome-wide approach

CANDI, ELEONORA;MELINO, GENNARO;
2006

Abstract

p63 is a developmentally regulated transcription factor related to p53. It is involved in the development of ectodermal tissues, including limb, skin and in general, multilayered epithelia. The DeltaNp63alpha isoform is thought to play a 'master' role in the asymmetric division of epithelial cells. It is also involved in the pathogenesis of several human diseases, phenotypically characterized by ectodermal dysplasia. Our understanding of transcriptional networks controlled by p63 is limited, owing to the low number of bona fide targets. To screen for new targets, we employed chromatin immunoprecipitation from keratinocytes (KCs) coupled to the microarray technology, using both CpG islands and promoter arrays. The former revealed 96 loci, the latter yielded 85 additional genes. We tested 40 of these targets in several functional assays, including: (i) in vivo binding by p63 in primary KCs; (ii) expression analysis in differentiating HaCaT cells and in cells overexpressing DeltaNp63alpha; (iii) promoter transactivation and (iv) immunostaining in normal tissues, confirming their regulation by p63. We discovered several new specific targets whose functional categorization links p63 to cell growth and differentiation.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11
English
Con Impact Factor ISI
ChIP on chip; p63; Skin; Targets
Vigano, M.A., Lamartine, J., Testoni, B., Merico, D., Alotto, D., Castagnoli, C., et al. (2006). New p63 targets in keratinocytes identified by a genome-wide approach. EMBO JOURNAL, 25(21), 5105-5116 [10.1038/sj.emboj.7601375].
Vigano, M; Lamartine, J; Testoni, B; Merico, D; Alotto, D; Castagnoli, C; Robert, A; Candi, E; Melino, G; Gidrol, X; Mantovani, R
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/34782
Citazioni
  • ???jsp.display-item.citation.pmc??? 56
  • Scopus 104
  • ???jsp.display-item.citation.isi??? 102
social impact