Proteomics is a leading technology for the study of proteins on a genome-wide scale, the objective of which is the large-scale identification of all protein species in a cell or tissue. In plant biology the key for successful proteomic application relies on the establishment of method to obtain high-quality protein samples. In this paper we compare three different procedures, TCA precipitation in acetone,TCA and PVPP precipitation in acetone, and precipitation in Tris-HCl to be applied for the extraction and purification of proteins from the aquatic moss Leptodictyum riparium . Better quality gels were obtained with the Tris-HCl extraction method.

Conte, B., Braglia, R., Basile, A., Forni, C. (2007). Proteomic and Bryophyte: comparison of different methods of protein extraction to study protein synthesis in the aquatic moss Leptodictyum riparium (Hedw.). CARYOLOGIA, 60(1-2), 102-105.

Proteomic and Bryophyte: comparison of different methods of protein extraction to study protein synthesis in the aquatic moss Leptodictyum riparium (Hedw.).

FORNI, CINZIA
2007-01-01

Abstract

Proteomics is a leading technology for the study of proteins on a genome-wide scale, the objective of which is the large-scale identification of all protein species in a cell or tissue. In plant biology the key for successful proteomic application relies on the establishment of method to obtain high-quality protein samples. In this paper we compare three different procedures, TCA precipitation in acetone,TCA and PVPP precipitation in acetone, and precipitation in Tris-HCl to be applied for the extraction and purification of proteins from the aquatic moss Leptodictyum riparium . Better quality gels were obtained with the Tris-HCl extraction method.
2007
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/01 - BOTANICA GENERALE
English
Con Impact Factor ISI
proteomics, Bryophytes, Leptodictyum riparium
http://www1.unifi.it/caryologia/past_volumes/60_1_2/60_1_sbi03.pdf
Conte, B., Braglia, R., Basile, A., Forni, C. (2007). Proteomic and Bryophyte: comparison of different methods of protein extraction to study protein synthesis in the aquatic moss Leptodictyum riparium (Hedw.). CARYOLOGIA, 60(1-2), 102-105.
Conte, B; Braglia, R; Basile, A; Forni, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/34777
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