Proteomics is a leading technology for the study of proteins on a genome-wide scale, the objective of which is the large-scale identification of all protein species in a cell or tissue. In plant biology the key for successful proteomic application relies on the establishment of method to obtain high-quality protein samples. In this paper we compare three different procedures, TCA precipitation in acetone,TCA and PVPP precipitation in acetone, and precipitation in Tris-HCl to be applied for the extraction and purification of proteins from the aquatic moss Leptodictyum riparium . Better quality gels were obtained with the Tris-HCl extraction method.
Conte, B., Braglia, R., Basile, A., Forni, C. (2007). Proteomic and Bryophyte: comparison of different methods of protein extraction to study protein synthesis in the aquatic moss Leptodictyum riparium (Hedw.). CARYOLOGIA, 60(1-2), 102-105.
Proteomic and Bryophyte: comparison of different methods of protein extraction to study protein synthesis in the aquatic moss Leptodictyum riparium (Hedw.).
FORNI, CINZIA
2007-01-01
Abstract
Proteomics is a leading technology for the study of proteins on a genome-wide scale, the objective of which is the large-scale identification of all protein species in a cell or tissue. In plant biology the key for successful proteomic application relies on the establishment of method to obtain high-quality protein samples. In this paper we compare three different procedures, TCA precipitation in acetone,TCA and PVPP precipitation in acetone, and precipitation in Tris-HCl to be applied for the extraction and purification of proteins from the aquatic moss Leptodictyum riparium . Better quality gels were obtained with the Tris-HCl extraction method.Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons
