Human endo-lysosomes possess a class of proteins called TPC channels on their membrane, which are essential for proper cell functioning. This protein family can be functionally studied by expressing them in plant vacuoles. Inhibition of hTPC activity by naringenin, one of the main flavonoids present in the human diet, has the potential to be beneficial in severe human diseases such as solid tumor development, melanoma, and viral infections. We attempted to identify the molecular basis of the interaction between hTPC2 and naringenin, using ensemble docking on molecular dynamics (MD) trajectories, but the specific binding site remains elusive, posing a challenge that could potentially be addressed in the future by increased computational power in MD and the combined use of microscopy techniques such as cryo-EM.

Minicozzi, V., Qi, T., Gradogna, A., Pozzolini, M., Milenkovic, S., Filippini, A., et al. (2023). A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas. BIOMOLECULAR CONCEPTS, 14(1) [10.1515/bmc-2022-0036].

A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas

Minicozzi V.
Membro del Collaboration Group
;
2023-01-01

Abstract

Human endo-lysosomes possess a class of proteins called TPC channels on their membrane, which are essential for proper cell functioning. This protein family can be functionally studied by expressing them in plant vacuoles. Inhibition of hTPC activity by naringenin, one of the main flavonoids present in the human diet, has the potential to be beneficial in severe human diseases such as solid tumor development, melanoma, and viral infections. We attempted to identify the molecular basis of the interaction between hTPC2 and naringenin, using ensemble docking on molecular dynamics (MD) trajectories, but the specific binding site remains elusive, posing a challenge that could potentially be addressed in the future by increased computational power in MD and the combined use of microscopy techniques such as cryo-EM.
2023
Pubblicato
Rilevanza internazionale
Review
Esperti anonimi
Settore FIS/07
Settore PHYS-06/A - Fisica per le scienze della vita, l'ambiente e i beni culturali
English
Senza Impact Factor ISI
flavonoids
naringenin
patch-clamp
plant vacuole
TPC channels
Minicozzi, V., Qi, T., Gradogna, A., Pozzolini, M., Milenkovic, S., Filippini, A., et al. (2023). A commentary on the inhibition of human TPC2 channel by the natural flavonoid naringenin: Methods, experiments, and ideas. BIOMOLECULAR CONCEPTS, 14(1) [10.1515/bmc-2022-0036].
Minicozzi, V; Qi, T; Gradogna, A; Pozzolini, M; Milenkovic, S; Filippini, A; Ceccarelli, M; Carpaneto, A
Articolo su rivista
File in questo prodotto:
File Dimensione Formato  
2023_BiomolecularConcepts_Naringenin.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Creative commons
Dimensione 1.33 MB
Formato Adobe PDF
1.33 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/347467
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact