Here we develop Lateral Flow Assays (LFAs) that employ as functional elements DNA-based structures decorated with reporter tags and recognition elements. We have rationally re-engineered tile-based DNA tubular structures that can act as scaffolds and can be decorated with recognition elements of different nature (i.e. antigens, aptamers or proteins) and with orthogonal fluorescent dyes. As a proof-of-principle we have developed sandwich and competitive multiplex lateral flow platforms for the detection of several targets, ranging from small molecules (digoxigenin, Dig and dinitrophenol, DNP), to antibodies (Anti-Dig, Anti-DNP and Anti-MUC1/EGFR bispecific antibodies) and proteins (thrombin). Coupling the advantages of functional DNA-based scaffolds together with the simplicity of LFAs, our approach offers the opportunity to detect a wide range of targets with nanomolar sensitivity and high specificity.

Brannetti, S., Gentile, S., Chamorro-Garcia, A., Barbero, L., Del Grosso, E., Ricci, F. (2023). Decorated DNA-based scaffolds as lateral flow biosensors. ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, 62(47) [10.1002/anie.202313243].

Decorated DNA-based scaffolds as lateral flow biosensors

Brannetti, Simone;Gentile, Serena;Chamorro-Garcia, Alejandro;Del Grosso, Erica
;
Ricci, Francesco
2023-11-20

Abstract

Here we develop Lateral Flow Assays (LFAs) that employ as functional elements DNA-based structures decorated with reporter tags and recognition elements. We have rationally re-engineered tile-based DNA tubular structures that can act as scaffolds and can be decorated with recognition elements of different nature (i.e. antigens, aptamers or proteins) and with orthogonal fluorescent dyes. As a proof-of-principle we have developed sandwich and competitive multiplex lateral flow platforms for the detection of several targets, ranging from small molecules (digoxigenin, Dig and dinitrophenol, DNP), to antibodies (Anti-Dig, Anti-DNP and Anti-MUC1/EGFR bispecific antibodies) and proteins (thrombin). Coupling the advantages of functional DNA-based scaffolds together with the simplicity of LFAs, our approach offers the opportunity to detect a wide range of targets with nanomolar sensitivity and high specificity.
20-nov-2023
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01
English
Con Impact Factor ISI
antibodies
DNA nanotechnology
DNA sensors
DNA structures
lateral flow assays
Brannetti, S., Gentile, S., Chamorro-Garcia, A., Barbero, L., Del Grosso, E., Ricci, F. (2023). Decorated DNA-based scaffolds as lateral flow biosensors. ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, 62(47) [10.1002/anie.202313243].
Brannetti, S; Gentile, S; Chamorro-Garcia, A; Barbero, L; Del Grosso, E; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/345404
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