The discovery of two new p53 homologs, p73 and p63, has defined a family of transcription factors heavily involved in the control of growth suppression, apoptosis, differentiation and development. While p53-deficient mice undergo spontaneous tumors, p73 and p63 knockout mice exhibit severe developmental defects. We demonstrate here that p73 gene is an in vivo transcriptional target of the muscle regulatory factors MyoD, myogenin, Myf5 and Myf6. Ectopic expression of the transcriptional repressor deltaEF1/ZEB/zfhx1a counteracts MyoD/Myf5- or MyoD/Myf6-mediated transcriptional activation of p73. A distinct pattern of in vivo recruitment of muscle regulatory factors and deltaEF1 on p73 regulatory regions was found between proliferating and differentiating muscle cells. We also found that deltaEF1 plays a role in the transcriptional regulation of p53 family members during keratinocytic differentiation. Mouse embryo fibroblasts derived from deltaEF1-deficient mice exhibit unbalanced expression of DeltaNp63, TAp73 and DeltaNp73 but not of TAp63 and p53. The analysis of tissues derived from deltaEF1+/- mice exhibit a selective enrichment of DeltaNp63 in skin.

Fontemaggi, G., Gurtner, A., Damalas, A., Costanzo, A., Higashi, Y., Sacchi, A., et al. (2005). deltaEF1 repressor controls selectively p53 family members during differentiation, 24(49), 7273-7280 [10.1038/sj.onc.1208891].

deltaEF1 repressor controls selectively p53 family members during differentiation

COSTANZO, ANTONIO;
2005-11-10

Abstract

The discovery of two new p53 homologs, p73 and p63, has defined a family of transcription factors heavily involved in the control of growth suppression, apoptosis, differentiation and development. While p53-deficient mice undergo spontaneous tumors, p73 and p63 knockout mice exhibit severe developmental defects. We demonstrate here that p73 gene is an in vivo transcriptional target of the muscle regulatory factors MyoD, myogenin, Myf5 and Myf6. Ectopic expression of the transcriptional repressor deltaEF1/ZEB/zfhx1a counteracts MyoD/Myf5- or MyoD/Myf6-mediated transcriptional activation of p73. A distinct pattern of in vivo recruitment of muscle regulatory factors and deltaEF1 on p73 regulatory regions was found between proliferating and differentiating muscle cells. We also found that deltaEF1 plays a role in the transcriptional regulation of p53 family members during keratinocytic differentiation. Mouse embryo fibroblasts derived from deltaEF1-deficient mice exhibit unbalanced expression of DeltaNp63, TAp73 and DeltaNp73 but not of TAp63 and p53. The analysis of tissues derived from deltaEF1+/- mice exhibit a selective enrichment of DeltaNp63 in skin.
10-nov-2005
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/35 - MALATTIE CUTANEE E VENEREE
English
Con Impact Factor ISI
Animals; Genes, Tumor Suppressor; Humans; Myogenic Regulatory Factor 5; Cell Proliferation; Myogenin; Fibroblasts; Mice, Knockout; Gene Expression Regulation, Neoplastic; Genes, p53; Nuclear Proteins; Phosphoproteins; Transcription Factors; Keratinocytes; Myogenic Regulatory Factors; Trans-Activators; Homeodomain Proteins; DNA-Binding Proteins; Cell Differentiation; Mice; MyoD Protein; Reverse Transcriptase Polymerase Chain Reaction; Tumor Suppressor Proteins; RNA, Messenger; Blotting, Western; Cells, Cultured; Zinc Fingers
Fontemaggi, G., Gurtner, A., Damalas, A., Costanzo, A., Higashi, Y., Sacchi, A., et al. (2005). deltaEF1 repressor controls selectively p53 family members during differentiation, 24(49), 7273-7280 [10.1038/sj.onc.1208891].
Fontemaggi, G; Gurtner, A; Damalas, A; Costanzo, A; Higashi, Y; Sacchi, A; Strano, S; Piaggio, G; Blandino, G
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/34268
Citazioni
  • ???jsp.display-item.citation.pmc??? 14
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 42
social impact