High contrast imaging of in vivo Chlorella sorokiniana cells with submicron spatial resolution was obtained with a contact water window X-ray microscopy technique using a point-like, laser-plasma produced, water-window X-ray radiation source, and LiF crystals as detectors. This novel type of X-ray imaging detectors is based on photoluminescence of stable electronic point defects, characterized by high intrinsic resolution. The fluorescence images obtained on LiF crystals exposed in single-shot experiments demonstrate the high sensitivity and dynamic range of this new detector. The powerful performances of LiF crystals allowed us to detect the exudates of Chlorella cells in their living medium and their spatial distribution in situ, without any special sample preparation.

Bonfigli, F., Faenov, A., Flora, F., Francucci, M., Gaudio, P., Lai, A., et al. (2008). High-resolution water window X-ray imaging of in vivo cells and their products using LiF crystal detectors. MICROSCOPY RESEARCH AND TECHNIQUE, 71(1), 35-41 [10.1002/jemt.20523].

High-resolution water window X-ray imaging of in vivo cells and their products using LiF crystal detectors

FRANCUCCI, MASSIMO;GAUDIO, PASQUALINO;MARTELLUCCI, SERGIO;RICHETTA, MARIA;
2008-01-01

Abstract

High contrast imaging of in vivo Chlorella sorokiniana cells with submicron spatial resolution was obtained with a contact water window X-ray microscopy technique using a point-like, laser-plasma produced, water-window X-ray radiation source, and LiF crystals as detectors. This novel type of X-ray imaging detectors is based on photoluminescence of stable electronic point defects, characterized by high intrinsic resolution. The fluorescence images obtained on LiF crystals exposed in single-shot experiments demonstrate the high sensitivity and dynamic range of this new detector. The powerful performances of LiF crystals allowed us to detect the exudates of Chlorella cells in their living medium and their spatial distribution in situ, without any special sample preparation.
gen-2008
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore FIS/01 - FISICA SPERIMENTALE
Settore FIS/02 - FISICA TEORICA, MODELLI E METODI MATEMATICI
English
Con Impact Factor ISI
Color centers; Imaging detectors; Lithium fluoride; X-ray microscopy
Bonfigli, F., Faenov, A., Flora, F., Francucci, M., Gaudio, P., Lai, A., et al. (2008). High-resolution water window X-ray imaging of in vivo cells and their products using LiF crystal detectors. MICROSCOPY RESEARCH AND TECHNIQUE, 71(1), 35-41 [10.1002/jemt.20523].
Bonfigli, F; Faenov, A; Flora, F; Francucci, M; Gaudio, P; Lai, A; Martellucci, S; Montereali, R; Pikuz, T; Reale, L; Richetta, M; Vincenti, M; Baldac...espandi
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/33788
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 30
  • ???jsp.display-item.citation.isi??? 28
social impact