Phylogenetic analysis and evaluation of drug-resistance were carried out upon 59 plasma samples from 58 treatment-naïve HIV-1 infected patients from Mozambique, enrolled in a free antiviral-therapy protocol in the frame of Drug-Resource-Enhancement against AIDS and Malnutrition (DREAM) programme. Sequencing of the first 1,300 bases of the pol-gene shows that all virus strains cluster within clade C, with the exception of a single patient carrying a G-subtype virus. Relevant mutations in the reverse transcriptase (RT) are rare: 118A/I/L/G (four patients), 179E/D/I (three patients), 333E/D (two patients), 101R, and 210F (one patient each). In Protease (PR), V82I (10.3%) is the only relevant mutation, while natural polymorphisms/secondary mutations are found, some at very high frequency: 20R (25.9%), 36I (91.4%), 36L (8.6%), 60E (31.0%), 63P (29.3%), and 93L (96.6%). Among them, mutations with a frequency >25% were further investigated to assess their covariation pattern with PI resistance associated mutations. The pattern of covariation observed for K20R and D60E (but not L63P and M36I) was different between C and B subtype isolates from PR-inhibitor-treated patients. The sequences were also analyzed to calculate the ratio of non-synonymous to synonymous substitution. The ratio for PR and RT was 0.116 and 0.093, respectively, suggesting a greater conservation in RT than PR in both subtypes B and C HIV strains. Taken together, the results demonstrate a consistent clade-homogeneity of viral strains circulating in Mozambique, and the very limited presence, in drug-naïve patients, of mutations associated with resistance to RT-inhibitors. The high frequency of secondary mutations/polymorphisms in HIV-PR deserves further studies to evaluate its relevance in clinical settings. © 2005 Wiley-Liss, Inc.
Bellocchi, M.C., Forbici, F., Palombi, L., Gori, C., Coelho, E., Svicher, V., et al. (2005). Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: Results from the DREAM programme. JOURNAL OF MEDICAL VIROLOGY, 76(4), 452-458.
Tipologia: | Articolo su rivista |
Citazione: | Bellocchi, M.C., Forbici, F., Palombi, L., Gori, C., Coelho, E., Svicher, V., et al. (2005). Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: Results from the DREAM programme. JOURNAL OF MEDICAL VIROLOGY, 76(4), 452-458. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore MED/42 - Igiene Generale e Applicata Settore MED/07 - Microbiologia e Microbiologia Clinica |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/jmv.20382 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | ago-2005 |
Titolo: | Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: Results from the DREAM programme |
Autori: | |
Autori: | Bellocchi, MC; Forbici, F; Palombi, L; Gori, C; Coelho, E; Svicher, V; D'Arrigo, R; Emberti, L; Ceffa, S; Erba, F; Marazzi, MC; Silberstein, FC; Perno, CF; |
Appare nelle tipologie: | 01 - Articolo su rivista |