In the last two decades, considerable progress has been made in the derivation of mammalian germ cells from pluripotent stem cells such as Embryonic Stem Cells (ESCs) and induced Pluripotent Stem Cells (iPSCs). The pluripotent stem cells are generally first induced into pre-gastrulating endoderm/mesoderm-like status and then specified into putative primordial germ cells (PGCs) termed PGC-like cells (PGCLCs) which possess the potential to generate oocytes and sperms. Adipose-derived mesenchymal stromal cells (ASCs) are multipotent cells, having the capacity to differentiate into cell types such as adipocytes, osteocytes and chondrocytes. Since no information is available about the capability of female human ASCs (hASCs) to generate PGCLCs, we compared protocols to produce such cells from hASCs themselves or from hASC-derived iPSCs. The results showed that, providing pre-induction into a peri-gastrulating endoderm/mesoderm-like status, hASCs can generate PGCLCs. This process, however, shows a lower efficiency than when hASC-derived iPSCs are used as starting cells. Although hASCs possess multipotency and express mesodermal genes, direct induction into PGCLCs resulted less efficient.

Salvatore, G., Dolci, S., Camaioni, A., Klinger, F.g., De Felici, M. (2023). Reprogramming human female adipose mesenchymal stem cells into primordial germ cell-like cells. STEM CELL REVIEWS AND REPORTS, 19, 2274-2283 [10.1007/s12015-023-10561-x].

Reprogramming human female adipose mesenchymal stem cells into primordial germ cell-like cells

Dolci, Susanna;Camaioni, Antonella;Klinger, Francesca Gioia;De Felici, Massimo
2023-06-20

Abstract

In the last two decades, considerable progress has been made in the derivation of mammalian germ cells from pluripotent stem cells such as Embryonic Stem Cells (ESCs) and induced Pluripotent Stem Cells (iPSCs). The pluripotent stem cells are generally first induced into pre-gastrulating endoderm/mesoderm-like status and then specified into putative primordial germ cells (PGCs) termed PGC-like cells (PGCLCs) which possess the potential to generate oocytes and sperms. Adipose-derived mesenchymal stromal cells (ASCs) are multipotent cells, having the capacity to differentiate into cell types such as adipocytes, osteocytes and chondrocytes. Since no information is available about the capability of female human ASCs (hASCs) to generate PGCLCs, we compared protocols to produce such cells from hASCs themselves or from hASC-derived iPSCs. The results showed that, providing pre-induction into a peri-gastrulating endoderm/mesoderm-like status, hASCs can generate PGCLCs. This process, however, shows a lower efficiency than when hASC-derived iPSCs are used as starting cells. Although hASCs possess multipotency and express mesodermal genes, direct induction into PGCLCs resulted less efficient.
20-giu-2023
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/17 - ISTOLOGIA
English
Adipose-derived mesenchymal stromal cells
Artificial gametes
Human infertility
PGCLCs
Primordial germ cells
Salvatore, G., Dolci, S., Camaioni, A., Klinger, F.g., De Felici, M. (2023). Reprogramming human female adipose mesenchymal stem cells into primordial germ cell-like cells. STEM CELL REVIEWS AND REPORTS, 19, 2274-2283 [10.1007/s12015-023-10561-x].
Salvatore, G; Dolci, S; Camaioni, A; Klinger, Fg; De Felici, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/330624
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