This study is a critical analysis of certain amplification assays for detecting Chlamydia trachomatis and Neisseria gonorrhoeae infections which have demonstrated that the plasmid-free variant of C. trachomatis is frequently responsible for infection in our patients. Specifically, we evaluated the performance of the strand displacement amplification (SDA) assay in detecting either C. trachomatis or N. gonorrhoeae in 1,190 clinical samples, both urogenital and ocular, from 1,005 consecutive patients. The results obtained with the BDProbeTec (TM) ET System were compared with three referenced amplification methods for C. trachomatis (detecting the 16S rRNA gene, the omp1 gene and the plasmid of C. trachomatis) and with both the culture method as well as an amplification assay followed by genetic identification performed using the Microseq 500 16S ribosomal DNA-based system for N. gonorrhoeae. The sensitivity of SDA (76%) in detecting C. trachomatis is significantly low when compared with that of other molecular techniques employing 16S rDNA or omp1 as a target. The specificity of the methods for detecting C. trachomatis was excellent, ranging from 99.4 to 100%. Furthermore, the results of SDA in detecting N. gonorrhoeae also provided excellent results (100% specificity and sensitivity).

Fontana, C., Favaro, M., Cicchetti, O., Minelli, S., Pistoia, E.S., & Favalli, C. (2005). Performance of strand displacement amplification assay in the detection of Chlamydia trachomatis and Neisseria gonorrhoeae. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 58(5), 283-288.

Performance of strand displacement amplification assay in the detection of Chlamydia trachomatis and Neisseria gonorrhoeae

FAVARO, MARCO;FAVALLI, CARTESIO
2005

Abstract

This study is a critical analysis of certain amplification assays for detecting Chlamydia trachomatis and Neisseria gonorrhoeae infections which have demonstrated that the plasmid-free variant of C. trachomatis is frequently responsible for infection in our patients. Specifically, we evaluated the performance of the strand displacement amplification (SDA) assay in detecting either C. trachomatis or N. gonorrhoeae in 1,190 clinical samples, both urogenital and ocular, from 1,005 consecutive patients. The results obtained with the BDProbeTec (TM) ET System were compared with three referenced amplification methods for C. trachomatis (detecting the 16S rRNA gene, the omp1 gene and the plasmid of C. trachomatis) and with both the culture method as well as an amplification assay followed by genetic identification performed using the Microseq 500 16S ribosomal DNA-based system for N. gonorrhoeae. The sensitivity of SDA (76%) in detecting C. trachomatis is significantly low when compared with that of other molecular techniques employing 16S rDNA or omp1 as a target. The specificity of the methods for detecting C. trachomatis was excellent, ranging from 99.4 to 100%. Furthermore, the results of SDA in detecting N. gonorrhoeae also provided excellent results (100% specificity and sensitivity).
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - Microbiologia e Microbiologia Clinica
English
Con Impact Factor ISI
bacterial protein; DNA 16S; RNA 16S; adult; analytical equipment; article; bacterium culture; bacterium detection; bioassay; Chlamydia trachomatis; chlamydiasis; controlled study; female; gene amplification; genetic identification; gonorrhea; human; intermethod comparison; major clinical study; male; Neisseria gonorrhoeae; nonhuman; performance; plasmid; sampling; sensitivity and specificity; urogenital system; visual system; Adult; Chlamydia Infections; Chlamydia trachomatis; DNA, Bacterial; DNA, Ribosomal; Eye; Female; Gonorrhea; Humans; Male; Middle Aged; Neisseria gonorrhoeae; Nucleic Acid Amplification Techniques; RNA, Bacterial; RNA, Ribosomal, 16S; Sensitivity and Specificity; Urogenital System
Fontana, C., Favaro, M., Cicchetti, O., Minelli, S., Pistoia, E.S., & Favalli, C. (2005). Performance of strand displacement amplification assay in the detection of Chlamydia trachomatis and Neisseria gonorrhoeae. JAPANESE JOURNAL OF INFECTIOUS DISEASES, 58(5), 283-288.
Fontana, C; Favaro, M; Cicchetti, O; Minelli, S; Pistoia, E; Favalli, C
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/32849
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