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EnglishDetailed knowledge of the influence of various parameters on macromolecular solubility is essential for crystallization. The concept of so-called 'ionic strength reducers' provides insight into the changes in solubility induced by organic solvents and hydrophilic polymers in aqueous electrolytic solutions. A simple and efficient procedure is presented which exploits the properties of ionic strength reducers in the purification and crystallization of proteins. Using two designed variants of the Rop protein as model systems, superior crystals have been obtained compared with conventional techniques. This procedure is particularly useful in cases where excessive nucleation leads to the growth of a large number of tiny crystals that are useless for crystallographic analysis.
Papanikolau, Y., Kotsifaki, D., Fadouloglou, V.E., Gazi, A.D., Glykos, N.M., Cesareni, G., et al. (2004). Ionic strength reducers: an efficient approach to protein purification and crystallization. Application to two Rop variants. ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, 60(7), 1334-1337 [10.1107/S0907444904008546].
| Tipologia: | Articolo su rivista | |
| Citazione: | Papanikolau, Y., Kotsifaki, D., Fadouloglou, V.E., Gazi, A.D., Glykos, N.M., Cesareni, G., et al. (2004). Ionic strength reducers: an efficient approach to protein purification and crystallization. Application to two Rop variants. ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, 60(7), 1334-1337 [10.1107/S0907444904008546]. | |
| IF: | Con Impact Factor ISI | |
| Lingua: | English | |
| Settore Scientifico Disciplinare: | Settore BIO/18 - Genetica | |
| Revisione (peer review): | Sì, ma tipo non specificato | |
| Tipo: | Articolo | |
| Rilevanza: | Rilevanza internazionale | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1107/S0907444904008546 | |
| Stato di pubblicazione: | Pubblicato | |
| Data di pubblicazione: | 2004 | |
| Titolo: | Ionic strength reducers: an efficient approach to protein purification and crystallization. Application to two Rop variants | |
| Autori: | ||
| Autori: | Papanikolau, Y; Kotsifaki, D; Fadouloglou, VE; Gazi, AD; Glykos, NM; Cesareni, G; Kokkinidis, M | |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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