In a previous work we demonstrated that extracellular adenosine-5'-triphosphate (ATP), acting on P2 receptors, exerts neuritogenic and trophic effects on the phaeochromocytoma PC12 cell line. These actions are comparable to those sustained by nerve growth factor (NGF) and involve several overlapping pathways. In this work, we describe some of the mechanisms recruited by ATP and NGF in maintaining PC12 cell survival after serum deprivation. We show that both ATP and NGF upregulate the expression of the stress-induced heat shock protein HSP70 and HSP90, whilst glucose-response protein GRP75 and GRP78 are not affected. In parallel with NGF, ATP prevents the cleavage and activation of caspase-2 and inhibits the release of cytochrome c from mitochondria into the cytoplasm. Finally, neither NGF, nor ATP directly modulate the expression of P2 receptors in the induction of cell survival. Our data contribute to dissect the biological mechanisms activated by extracellular purines exerting trophic actions and to establish that survival and neurite outgrowth lie on different mechanistic pathways.

D'Ambrosi, N., Murra, B., Vacca, F., Volonté, C. (2004). Pathways of survival induced by NGF and extracellular ATP after growth factor deprivation, 146, 93-100 [10.1016/S0079-6123(03)46006-8].

Pathways of survival induced by NGF and extracellular ATP after growth factor deprivation

D'Ambrosi, Nadia;Vacca, Fabrizio;
2004-01-01

Abstract

In a previous work we demonstrated that extracellular adenosine-5'-triphosphate (ATP), acting on P2 receptors, exerts neuritogenic and trophic effects on the phaeochromocytoma PC12 cell line. These actions are comparable to those sustained by nerve growth factor (NGF) and involve several overlapping pathways. In this work, we describe some of the mechanisms recruited by ATP and NGF in maintaining PC12 cell survival after serum deprivation. We show that both ATP and NGF upregulate the expression of the stress-induced heat shock protein HSP70 and HSP90, whilst glucose-response protein GRP75 and GRP78 are not affected. In parallel with NGF, ATP prevents the cleavage and activation of caspase-2 and inhibits the release of cytochrome c from mitochondria into the cytoplasm. Finally, neither NGF, nor ATP directly modulate the expression of P2 receptors in the induction of cell survival. Our data contribute to dissect the biological mechanisms activated by extracellular purines exerting trophic actions and to establish that survival and neurite outgrowth lie on different mechanistic pathways.
2004
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
P2 receptor
PC12 cells
caspase-2
cytochrome c
heat shock proteins
D'Ambrosi, N., Murra, B., Vacca, F., Volonté, C. (2004). Pathways of survival induced by NGF and extracellular ATP after growth factor deprivation, 146, 93-100 [10.1016/S0079-6123(03)46006-8].
D'Ambrosi, N; Murra, B; Vacca, F; Volonté, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/324767
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