In this study, for the first time, the anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial property of P. atlantica Desf. subsp. atlantica leaf-bud extract have been investigated. The anti-inflammatory activity was determined in vivo by reducing carrageenan-induced hind paw edema in mice, while the antiradical function was evaluated using DPPH, total antioxidant capacity (TAC) and reduction power assays. The extract induced a significant reduction of the edema, from 1 to 6 h in a dose-dependent manner (150, 200 and 300 mg/kg). Histological observations of the inflamed tissues also confirmed this. An effective antioxidant activity of the plant samples was demonstrated, showing an EC50 = 0.183 ± 0.005 mg/mL for the DPPH test, a value of 28.776 ± 2.541 mg AAE/g for the TAC and an EC50 = 0.136 ± 0.003 mg/mL for reducing power. The leaf-bud extract also revealed a good antimicrobial activity against S. aureus and L. monocytogenes (mean diameter of inhibition zones of 13.2 and 17.0 mm, respectively), while a slight antifungal effect was observed. The plant preparation was then documented to inhibit tyrosinase activity, with an EC50 value of 0.098 ± 0.00 mg/mL in a dose-dependent manner. HPLC–DAD analysis revealed that dimethyl-allyl caffeic acid and rutin were the most abundant molecules. The current data documented that P. atlantica leaf-bud extract has strong biological properties and constitutes a potential source of pharmacological molecules.

Benmahieddine, A., Belyagoubi-Benhammou, N., Belyagoubi, L., Amari, N.o., Zerey-Belaskri, A.e., Gismondi, A., et al. (2023). Leaf-buds of Pistacia atlantica: a novel source of bioactive molecules with high anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial properties. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 29(2), 209-219 [10.1007/s12298-023-01290-z].

Leaf-buds of Pistacia atlantica: a novel source of bioactive molecules with high anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial properties

Gismondi A.;Di Marco G.;Canini A.;
2023-01-01

Abstract

In this study, for the first time, the anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial property of P. atlantica Desf. subsp. atlantica leaf-bud extract have been investigated. The anti-inflammatory activity was determined in vivo by reducing carrageenan-induced hind paw edema in mice, while the antiradical function was evaluated using DPPH, total antioxidant capacity (TAC) and reduction power assays. The extract induced a significant reduction of the edema, from 1 to 6 h in a dose-dependent manner (150, 200 and 300 mg/kg). Histological observations of the inflamed tissues also confirmed this. An effective antioxidant activity of the plant samples was demonstrated, showing an EC50 = 0.183 ± 0.005 mg/mL for the DPPH test, a value of 28.776 ± 2.541 mg AAE/g for the TAC and an EC50 = 0.136 ± 0.003 mg/mL for reducing power. The leaf-bud extract also revealed a good antimicrobial activity against S. aureus and L. monocytogenes (mean diameter of inhibition zones of 13.2 and 17.0 mm, respectively), while a slight antifungal effect was observed. The plant preparation was then documented to inhibit tyrosinase activity, with an EC50 value of 0.098 ± 0.00 mg/mL in a dose-dependent manner. HPLC–DAD analysis revealed that dimethyl-allyl caffeic acid and rutin were the most abundant molecules. The current data documented that P. atlantica leaf-bud extract has strong biological properties and constitutes a potential source of pharmacological molecules.
2023
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/01 - BOTANICA GENERALE
Settore BIO/04
Settore BIO/10
Settore BIO/11
Settore BIO/14
English
Con Impact Factor ISI
Biological activities
HPLC–DAD
Hydro-methanolic extract
Leaf-bud
Phenolic compound
Pistacia atlantica
Benmahieddine, A., Belyagoubi-Benhammou, N., Belyagoubi, L., Amari, N.o., Zerey-Belaskri, A.e., Gismondi, A., et al. (2023). Leaf-buds of Pistacia atlantica: a novel source of bioactive molecules with high anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial properties. PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS, 29(2), 209-219 [10.1007/s12298-023-01290-z].
Benmahieddine, A; Belyagoubi-Benhammou, N; Belyagoubi, L; Amari, No; Zerey-Belaskri, Ae; Gismondi, A; Di Marco, G; Canini, A; Habi, S; Atik Bekkara, F...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/324004
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