Multiparametric flow cytometry is an extensively used technique to assess the presence of different cellular populations in immunology and hematology. During routine immunophenotyping analysis, it is not uncommon to face cells of non-hemopoietic origin, negative for CD45 and other myeloid, megakaryocytic, B and T lineage antigens and positive for at least one antibody among CD56, CD117 and CD138. If cytology cannot identify cell origin, especially in cases of unclear interpretation, the contribution of multiparametric flow cytometry analysis can be crucial. We report 6 patients with a clinical suspicion of hematological disease in which multiparametric flow cytometry was extremely useful to quickly exclude blood disorders in order to initiate patients to the most appropriate diagnostic process.

Bisegna, M.l., Cordone, I., Peragine, N., Milani, M.l., Intoppa, S., de Fabritiis, P., et al. (2023). Neoplastic bone marrow invasion:rapid exclusion of hematological disease by flow cytometric routine panels. BLOOD CELLS, MOLECULES, & DISEASES, 99, 102721 [10.1016/j.bcmd.2022.102721].

Neoplastic bone marrow invasion:rapid exclusion of hematological disease by flow cytometric routine panels

de Fabritiis, Paolo;
2023-03-01

Abstract

Multiparametric flow cytometry is an extensively used technique to assess the presence of different cellular populations in immunology and hematology. During routine immunophenotyping analysis, it is not uncommon to face cells of non-hemopoietic origin, negative for CD45 and other myeloid, megakaryocytic, B and T lineage antigens and positive for at least one antibody among CD56, CD117 and CD138. If cytology cannot identify cell origin, especially in cases of unclear interpretation, the contribution of multiparametric flow cytometry analysis can be crucial. We report 6 patients with a clinical suspicion of hematological disease in which multiparametric flow cytometry was extremely useful to quickly exclude blood disorders in order to initiate patients to the most appropriate diagnostic process.
mar-2023
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/15 - MALATTIE DEL SANGUE
English
Bone marrow invasion
Multiparametric flow cytometry
Non-hematological disease
Oncology
Bisegna, M.l., Cordone, I., Peragine, N., Milani, M.l., Intoppa, S., de Fabritiis, P., et al. (2023). Neoplastic bone marrow invasion:rapid exclusion of hematological disease by flow cytometric routine panels. BLOOD CELLS, MOLECULES, & DISEASES, 99, 102721 [10.1016/j.bcmd.2022.102721].
Bisegna, Ml; Cordone, I; Peragine, N; Milani, Ml; Intoppa, S; de Fabritiis, P; Martelli, M; De Propris, Ms
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/318978
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