Fibrillarin is one of the protein components that together with U3 snRNA constitute the U3 snRNP, a small nuclear ribonucleoprotein particle involved in ribosomal RNA processing in eucaryotic cells. Using an antifibrillarin antiserum for protein detection and a fibrillarin cDNA and a synthetic oligonucleotide complementary to U3 snRNA as hybridization probes, the expression of these two components has been studied during Xenopus development. Fibrillarin mRNA is accumulated early in oogenesis, like many other messengers, and translated during oocyte growth. Fibrillarin protein is thus progressively accumulated throughout oogenesis to be assembled with U3 snRNA and used for ribosome production in the amplified nucleoli. After fertilization, the amount of U3 snRNA decreases while the maternally accumulated fibrillarin mRNA is maintained and utilized to produce more protein. After the mid-blastula transition, stored fibrillarin is assembled with newly synthesized U3 snRNA and becomes localized in the prenucleolar bodies and reforming nucleoli.

Caizergues-Ferrer, M., Mathieu, C., Mariottini, P., Amalric, F., & Amaldi, F. (1991). Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis. DEVELOPMENT, 112(1), 317-326.

Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis

AMALDI, FRANCESCO
1991

Abstract

Fibrillarin is one of the protein components that together with U3 snRNA constitute the U3 snRNP, a small nuclear ribonucleoprotein particle involved in ribosomal RNA processing in eucaryotic cells. Using an antifibrillarin antiserum for protein detection and a fibrillarin cDNA and a synthetic oligonucleotide complementary to U3 snRNA as hybridization probes, the expression of these two components has been studied during Xenopus development. Fibrillarin mRNA is accumulated early in oogenesis, like many other messengers, and translated during oocyte growth. Fibrillarin protein is thus progressively accumulated throughout oogenesis to be assembled with U3 snRNA and used for ribosome production in the amplified nucleoli. After fertilization, the amount of U3 snRNA decreases while the maternally accumulated fibrillarin mRNA is maintained and utilized to produce more protein. After the mid-blastula transition, stored fibrillarin is assembled with newly synthesized U3 snRNA and becomes localized in the prenucleolar bodies and reforming nucleoli.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/11
English
Con Impact Factor ISI
FIBRILLARIN; U3 SNRNA; GENE EXPRESSION; XENOPUS-LAEVIS; DEVELOPMENT
10
Caizergues-Ferrer, M., Mathieu, C., Mariottini, P., Amalric, F., & Amaldi, F. (1991). Developmental expression of fibrillarin and U3 snRNA in Xenopus laevis. DEVELOPMENT, 112(1), 317-326.
Caizergues Ferrer, M; Mathieu, C; Mariottini, P; Amalric, F; Amaldi, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/31867
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