The catalysis of class III plant peroxidases is described based on the reaction scheme of horseradish peroxidase. The mechanism consists in four distinct steps: (a) binding of peroxide to the heme-FeIII to form a very unstable peroxide complex, Compound 0; (b) oxidation of the iron to generate Compound I, a ferryl species with a π-cation radical in the porphyrin ring; (c) reduction of Compound I by one substrate molecule to produce a substrate radical and another ferryl species, Compound II; (d) reduction of Compound II by a second substrate molecule to release a second substrate radical and regenerate the native enzyme. Under unfavourable conditions some inactive enzyme species can be formed, known as dead-end species. Two calcium ions are normally found in plant peroxidases and appear to be important for the catalytic efficiency.

Longu, S., Medda, R., Padiglia, A., Pedersen, J.z., Floris, G. (2004). The reaction mechanism of plant peroxidases. ITALIAN JOURNAL OF BIOCHEMISTRY, 53, 42-46.

The reaction mechanism of plant peroxidases.

PEDERSEN, JENS ZACHO;
2004-01-01

Abstract

The catalysis of class III plant peroxidases is described based on the reaction scheme of horseradish peroxidase. The mechanism consists in four distinct steps: (a) binding of peroxide to the heme-FeIII to form a very unstable peroxide complex, Compound 0; (b) oxidation of the iron to generate Compound I, a ferryl species with a π-cation radical in the porphyrin ring; (c) reduction of Compound I by one substrate molecule to produce a substrate radical and another ferryl species, Compound II; (d) reduction of Compound II by a second substrate molecule to release a second substrate radical and regenerate the native enzyme. Under unfavourable conditions some inactive enzyme species can be formed, known as dead-end species. Two calcium ions are normally found in plant peroxidases and appear to be important for the catalytic efficiency.
2004
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Peroxidase; iron; hydrogen peroxide; calcium ions
http://www.ncbi.nlm.nih.gov/pubmed/15356961
Longu, S., Medda, R., Padiglia, A., Pedersen, J.z., Floris, G. (2004). The reaction mechanism of plant peroxidases. ITALIAN JOURNAL OF BIOCHEMISTRY, 53, 42-46.
Longu, S; Medda, R; Padiglia, A; Pedersen, Jz; Floris, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/31846
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