: Gene-environment interactions can perturb the epigenome, triggering network alterations that participate in cancer pathogenesis. Integrating epigenomics, transcriptomics, and metabolic analyses with functional perturbation, we show that the tumor suppressor p53 preserves genomic integrity by empowering adequate levels of the universal methyl donor S-adenosylmethionine (SAM). In p53-deficient cells, perturbation of DNA methylation promotes derepression of heterochromatin, massive loss of histone H3-lysine 9 methylation, and consequent upregulation of satellite RNAs that triggers R-loop-associated replication stress and chromosomal aberrations. In p53-deficient cells, the inadequate SAM level underlies the inability to respond to perturbation because exogenous reintroduction of SAM represses satellite elements and restores the ability to cope with stress. Mechanistically, p53 transcriptionally controls genes involved in one-carbon metabolism, including Slc43a2, the methionine uptake transporter that is critical for SAM synthesis. Supported by clinical data, our findings shed light on the role of p53-mediated metabolism in preventing unscheduled R-loop-associated genomic instability.

Panatta, E., Butera, A., Mammarella, E., Pitolli, C., Mauriello, A., Leist, M., et al. (2022). Metabolic regulation by p53 prevents R-loop-associated genomic instability. CELL REPORTS, 41(5), 111568 [10.1016/j.celrep.2022.111568].

Metabolic regulation by p53 prevents R-loop-associated genomic instability

Panatta E.;Pitolli C.;Mauriello A.;Melino G.;Amelio I.
2022-01-01

Abstract

: Gene-environment interactions can perturb the epigenome, triggering network alterations that participate in cancer pathogenesis. Integrating epigenomics, transcriptomics, and metabolic analyses with functional perturbation, we show that the tumor suppressor p53 preserves genomic integrity by empowering adequate levels of the universal methyl donor S-adenosylmethionine (SAM). In p53-deficient cells, perturbation of DNA methylation promotes derepression of heterochromatin, massive loss of histone H3-lysine 9 methylation, and consequent upregulation of satellite RNAs that triggers R-loop-associated replication stress and chromosomal aberrations. In p53-deficient cells, the inadequate SAM level underlies the inability to respond to perturbation because exogenous reintroduction of SAM represses satellite elements and restores the ability to cope with stress. Mechanistically, p53 transcriptionally controls genes involved in one-carbon metabolism, including Slc43a2, the methionine uptake transporter that is critical for SAM synthesis. Supported by clinical data, our findings shed light on the role of p53-mediated metabolism in preventing unscheduled R-loop-associated genomic instability.
2022
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/08 - ANATOMIA PATOLOGICA
Settore BIO/10 - BIOCHIMICA
Settore BIO/11 - BIOLOGIA MOLECOLARE
English
CP: Molecular biology
cancer
chromosome stability
epigenetic integrity
p53
tumor suppression
Panatta, E., Butera, A., Mammarella, E., Pitolli, C., Mauriello, A., Leist, M., et al. (2022). Metabolic regulation by p53 prevents R-loop-associated genomic instability. CELL REPORTS, 41(5), 111568 [10.1016/j.celrep.2022.111568].
Panatta, E; Butera, A; Mammarella, E; Pitolli, C; Mauriello, A; Leist, M; Knight, Ra; Melino, G; Amelio, I
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/316785
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