The effect of dietary copper (Cu) levels on growth, oxidative stress, fatty acid profile and bone health of gilthead seabream larvae (Sparus aurata) was studied in 47 days-post-hatching fish fed 4 experimental diets containing 17, 18, 19 and 25 mg Cu/kg diet. After 21 days of feeding, larval growth and survival were not affected by the dietary Cu levels. However, larvae fed the non-supplemented diet (17 mg Cu/kg) showed clear symptoms of Cu deficiency, including increased lipid peroxidation and reduced bone mineralization. Increased peroxidation risk caused a reduction in essential fatty acids and n-3 PUFA (polyunsaturated fatty acids) contents in whole body lipids, whereas the poor vertebral body mineralization in seabream larvae was related to a marked increase in abdominal vertebrae anomalies. On the contrary, dietary Cu supplementation raised whole-body contents in DHA, EPA and n-3 PUFA and reduced MUFA, TBARS and the incidence of severe, cranial, branchiostegal rays and abdominal vertebral anomalies. Polynomial regression models applied to all these parameters suggested optimum dietary Cu levels to be 21.5–22.6 mg/kg in gilthead seabream larvae microdiets supplemented with CuSO4.5H2O. These levels (21.5–22.6 mg/kg) are in the range of the Cu contents in copepods (12–38 mg/kg) and on the verge of the dietary levels authorized by EU Commission in feeds for cultured fish species (25 mg Cu/kg diet).

Tseng, Y., Eryalçın, K.m., Sivagurunathan, U., Domínguez, D., Hernández-Cruz, C.m., Boglione, C., et al. (2023). Effects of the dietary supplementation of copper on growth, oxidative stress, fatty acid profile and skeletal development in gilthead seabream (Sparus aurata) larvae. AQUACULTURE [10.1016/j.aquaculture.2023.739319].

Effects of the dietary supplementation of copper on growth, oxidative stress, fatty acid profile and skeletal development in gilthead seabream (Sparus aurata) larvae

Boglione, C.;
2023-01-31

Abstract

The effect of dietary copper (Cu) levels on growth, oxidative stress, fatty acid profile and bone health of gilthead seabream larvae (Sparus aurata) was studied in 47 days-post-hatching fish fed 4 experimental diets containing 17, 18, 19 and 25 mg Cu/kg diet. After 21 days of feeding, larval growth and survival were not affected by the dietary Cu levels. However, larvae fed the non-supplemented diet (17 mg Cu/kg) showed clear symptoms of Cu deficiency, including increased lipid peroxidation and reduced bone mineralization. Increased peroxidation risk caused a reduction in essential fatty acids and n-3 PUFA (polyunsaturated fatty acids) contents in whole body lipids, whereas the poor vertebral body mineralization in seabream larvae was related to a marked increase in abdominal vertebrae anomalies. On the contrary, dietary Cu supplementation raised whole-body contents in DHA, EPA and n-3 PUFA and reduced MUFA, TBARS and the incidence of severe, cranial, branchiostegal rays and abdominal vertebral anomalies. Polynomial regression models applied to all these parameters suggested optimum dietary Cu levels to be 21.5–22.6 mg/kg in gilthead seabream larvae microdiets supplemented with CuSO4.5H2O. These levels (21.5–22.6 mg/kg) are in the range of the Cu contents in copepods (12–38 mg/kg) and on the verge of the dietary levels authorized by EU Commission in feeds for cultured fish species (25 mg Cu/kg diet).
31-gen-2023
Online ahead of print
Rilevanza internazionale
Articolo
Esperti anonimi
Settore AGR/19 - ZOOTECNICA SPECIALE
Settore BIO/07 - ECOLOGIA
English
Con Impact Factor ISI
Gilthead seabream (Sparus aurata) larvae; bone health; Copper requirement
https://www.sciencedirect.com/science/article/pii/S0044848623000923
Tseng, Y., Eryalçın, K.m., Sivagurunathan, U., Domínguez, D., Hernández-Cruz, C.m., Boglione, C., et al. (2023). Effects of the dietary supplementation of copper on growth, oxidative stress, fatty acid profile and skeletal development in gilthead seabream (Sparus aurata) larvae. AQUACULTURE [10.1016/j.aquaculture.2023.739319].
Tseng, Y; Eryalçın, Km; Sivagurunathan, U; Domínguez, D; Hernández-Cruz, Cm; Boglione, C; Prabhu, Paj; Izquierdo, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/314538
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