The activation of telomerase in phytohemagglutinin-stimulated peripheral lymphocytes is thought to play a role in telomere maintenance and DNA repair. Considering the importance of this enzyme in both cancer and senescence, we studied the effects of the telomerase inhibitor 3'-azido-3'-deoxythymidine on the frequency of chromosomal aberrations and micronuclei induced in peripheral blood lymphocytes (PBL) of elderly monozygotic and dizygotic twins, evaluated with respect to the genotoxic effects induced in unrelated young subjects. Our results show that the cytogenetic damage induced by 3'-azido-3'-deoxythymidine in human PBL was mainly regulated by genetic factors and allowed the identification of hypersensitive subjects. Ageing, which did not modify the individual susceptibility to 3'-azido-3'-deoxythymidine induction of chromosome aberrations and micronuclei, nevertheless determined an overall increase in nuclear damage.

Caporossi, D., Argentin, G., Pittaluga, M., Parisi, P., Tedeschi, B., Vernole, P., et al. (2004). Individual susceptibility to DNA telomerase inhibitors: a study on the chromosome instability induced by 3 '-azido-3 '-deoxythymidine in lymphocytes of elderly twins. MUTAGENESIS, 19(2), 99-104 [10.1093/mutage/geh014].

Individual susceptibility to DNA telomerase inhibitors: a study on the chromosome instability induced by 3 '-azido-3 '-deoxythymidine in lymphocytes of elderly twins

ARGENTIN, GABRIELLA;VERNOLE, PATRIZIA;CICCHETTI, ROSADELE
2004-01-01

Abstract

The activation of telomerase in phytohemagglutinin-stimulated peripheral lymphocytes is thought to play a role in telomere maintenance and DNA repair. Considering the importance of this enzyme in both cancer and senescence, we studied the effects of the telomerase inhibitor 3'-azido-3'-deoxythymidine on the frequency of chromosomal aberrations and micronuclei induced in peripheral blood lymphocytes (PBL) of elderly monozygotic and dizygotic twins, evaluated with respect to the genotoxic effects induced in unrelated young subjects. Our results show that the cytogenetic damage induced by 3'-azido-3'-deoxythymidine in human PBL was mainly regulated by genetic factors and allowed the identification of hypersensitive subjects. Ageing, which did not modify the individual susceptibility to 3'-azido-3'-deoxythymidine induction of chromosome aberrations and micronuclei, nevertheless determined an overall increase in nuclear damage.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
DNA; phytohemagglutinin; telomerase inhibitor; zidovudine; adult; aged; aging; article; cell culture; chromosome aberration; chromosome analysis; chromosome breakage; controlled study; dizygotic twins; DNA repair; enzyme activation; genetic stability; genetic susceptibility; genotoxicity; human; human cell; lymphocyte; lymphocyte activation; male; monozygotic twins; priority journal; statistical analysis; telomere; Age Factors; Aged; Chromosomal Instability; DNA Damage; Humans; Lymphocytes; Male; Telomerase; Twins; Zidovudine
Caporossi, D., Argentin, G., Pittaluga, M., Parisi, P., Tedeschi, B., Vernole, P., et al. (2004). Individual susceptibility to DNA telomerase inhibitors: a study on the chromosome instability induced by 3 '-azido-3 '-deoxythymidine in lymphocytes of elderly twins. MUTAGENESIS, 19(2), 99-104 [10.1093/mutage/geh014].
Caporossi, D; Argentin, G; Pittaluga, M; Parisi, P; Tedeschi, B; Vernole, P; Cicchetti, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/31448
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