An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
Mayorga-Martinez, C.c., Chamorro-Garcia, A., Serrano, L., Rivas, L., Quesada-Gonzalez, D., Altet, L., et al. (2015). An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection. JOURNAL OF MATERIALS CHEMISTRY. B, 3(26), 5166-5171 [10.1039/c5tb00545k].
An iridium oxide nanoparticle and polythionine thin film based platform for sensitive Leishmania DNA detection
Chamorro-Garcia, Alejandro;
2015-07-14
Abstract
An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO2 NP). An aminated oligonucleotide probe is immobilized on the IrO2 NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.| File | Dimensione | Formato | |
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