A discordant response to highly active antiretroviral therapy (HAART) occurs when CD4 T cell counts are stable or increased over time despite persistently detectable HIV-RNA levels. In order to identify immunological factors affecting discordant treatment responses, a total of 27 HIV-infected patients were studied: (a) 10 naive patients (mean CD4(+) = 101.5 cells/mul; mean HIV-RNA = 4.8 log(10) copies/ml); (b) seven responder patients (mean CD4(+) = 908.9 cells/mul); and (c) 10 discordant patients (mean CD4(+) = 396.1 cells/mul; mean HIV-RNA = 5.4 log(10) copies/ml). Five healthy blood donors were included as HIV-seronegative controls. The following parameters were evaluated: interleukin (IL)-15 production by monocyte-derived dendritic cells (MDDC) after stimulation with lypopolysaccaride (LPS) and Candida albicans; recall and HIV-1-specific antigen lymphocyte proliferation (LP). Increased levels of IL-15 production by MDDC after stimulation with LPS and C. albicans were found both in discordant patients and responder patients. Conversely, a strong reduction of IL-15 levels was observed in naive patients. Discordant patients developed positive LP responses to C. albicans and HIV-1 p24. LP in response to C. albicans and HIV-1 p24 was also positive in responder patients. Decreased LP response was found in naive patients. In conclusion, HIV-infected patients with discordant viro-immunological responses to HAART present increased levels of IL-15 production by MDDC and enhanced recall and HIV-1-specific antigen LP responses, suggesting an improvement in indices of immune function.

D'Ettorre, G., Forcina, G., Andreotti, M., Sarmati, L., Palmisano, L., Andreoni, M., et al. (2004). Interleukin-15 production by monocyte-derived dendritic cells and T cell proliferation in HIV-infected patients with discordant response to highly active antiretroviral therapy. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 135(2), 280-285 [10.1111/j.1365-2249.2004.02373.x].

Interleukin-15 production by monocyte-derived dendritic cells and T cell proliferation in HIV-infected patients with discordant response to highly active antiretroviral therapy

SARMATI, LOREDANA;ANDREONI, MASSIMO;
2004

Abstract

A discordant response to highly active antiretroviral therapy (HAART) occurs when CD4 T cell counts are stable or increased over time despite persistently detectable HIV-RNA levels. In order to identify immunological factors affecting discordant treatment responses, a total of 27 HIV-infected patients were studied: (a) 10 naive patients (mean CD4(+) = 101.5 cells/mul; mean HIV-RNA = 4.8 log(10) copies/ml); (b) seven responder patients (mean CD4(+) = 908.9 cells/mul); and (c) 10 discordant patients (mean CD4(+) = 396.1 cells/mul; mean HIV-RNA = 5.4 log(10) copies/ml). Five healthy blood donors were included as HIV-seronegative controls. The following parameters were evaluated: interleukin (IL)-15 production by monocyte-derived dendritic cells (MDDC) after stimulation with lypopolysaccaride (LPS) and Candida albicans; recall and HIV-1-specific antigen lymphocyte proliferation (LP). Increased levels of IL-15 production by MDDC after stimulation with LPS and C. albicans were found both in discordant patients and responder patients. Conversely, a strong reduction of IL-15 levels was observed in naive patients. Discordant patients developed positive LP responses to C. albicans and HIV-1 p24. LP in response to C. albicans and HIV-1 p24 was also positive in responder patients. Decreased LP response was found in naive patients. In conclusion, HIV-infected patients with discordant viro-immunological responses to HAART present increased levels of IL-15 production by MDDC and enhanced recall and HIV-1-specific antigen LP responses, suggesting an improvement in indices of immune function.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/17 - Malattie Infettive
English
Con Impact Factor ISI
Dendritic cells; Discordant patients; HIV; Interleukin-15; T cells
D'Ettorre, G., Forcina, G., Andreotti, M., Sarmati, L., Palmisano, L., Andreoni, M., et al. (2004). Interleukin-15 production by monocyte-derived dendritic cells and T cell proliferation in HIV-infected patients with discordant response to highly active antiretroviral therapy. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 135(2), 280-285 [10.1111/j.1365-2249.2004.02373.x].
D'Ettorre, G; Forcina, G; Andreotti, M; Sarmati, L; Palmisano, L; Andreoni, M; Vella, S; Mastroianni, Cm; Vullo, V
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/31416
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