Several non-phagocytic cells can actively generate the superoxide anion by NAD(P)H oxidases resembling the enzymatic complex typical of phagocytes. Overexpression of periplasmic Cu,ZnSOD rescues invasive E. coli strains from killing within epithelial cells, suggesting that superoxide generation by such cells can oxidatively damage invading bacteria. Pre-treatment of HeLa cells with diphenyl iodonium or 4'-hydroxy-3'-methoxyacetophenone, two inhibitors of NAD(P)H oxidase, significantly enhances intracellular survival of wild type invasive E. coli cells. On the contrary, these inhibitors have no effect on the intracellular survival of an invasive E. coli strain engineered to overexpress Cu,ZnSOD. These results support the hypothesis that superoxide generation by a NAD(P)H oxidase-like complex can limit bacterial survival within epithelial cells and suggest that the role of periplasmic Cu,ZnSOD in bacterial infections is not simply that of conferring protection against the phagocytic oxidative burst.

Battistoni, A., Ajello, M., Ammendola, S., Superti, F., Rotilio, G., Valenti, P. (2004). Involvement of reactive oxygen species in bacterial killing within epithelial cells. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 17(1), 71-76.

Involvement of reactive oxygen species in bacterial killing within epithelial cells

Battistoni A;Ammendola S.;Rotilio G.;
2004-01-01

Abstract

Several non-phagocytic cells can actively generate the superoxide anion by NAD(P)H oxidases resembling the enzymatic complex typical of phagocytes. Overexpression of periplasmic Cu,ZnSOD rescues invasive E. coli strains from killing within epithelial cells, suggesting that superoxide generation by such cells can oxidatively damage invading bacteria. Pre-treatment of HeLa cells with diphenyl iodonium or 4'-hydroxy-3'-methoxyacetophenone, two inhibitors of NAD(P)H oxidase, significantly enhances intracellular survival of wild type invasive E. coli cells. On the contrary, these inhibitors have no effect on the intracellular survival of an invasive E. coli strain engineered to overexpress Cu,ZnSOD. These results support the hypothesis that superoxide generation by a NAD(P)H oxidase-like complex can limit bacterial survival within epithelial cells and suggest that the role of periplasmic Cu,ZnSOD in bacterial infections is not simply that of conferring protection against the phagocytic oxidative burst.
2004
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Antioxidant enzyme; Bacterial invasivity; Cu,Zn superoxide dismutase; Escherichia coli; NADPH oxidase; Oxygen radicals; sodC
http://www.ncbi.nlm.nih.gov/pubmed/15000869
Battistoni, A., Ajello, M., Ammendola, S., Superti, F., Rotilio, G., Valenti, P. (2004). Involvement of reactive oxygen species in bacterial killing within epithelial cells. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 17(1), 71-76.
Battistoni, A; Ajello, M; Ammendola, S; Superti, F; Rotilio, G; Valenti, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/31314
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