The quantitative detection of different molecular targets is of utmost importance for a variety of human activities, ranging from healthcare to environmental studies. Bioanalytical methods have been developed to solve this and to achieve the quantification of multiple targets from small volume samples. Generally, they can be divided into two different classes: point of care (PoC) and laboratory-based approaches. The former is rapid, low-cost, and user-friendly; however, the majority of the tests are semiquantitative, lacking in specificity and sensitivity. On the contrary, laboratory-based approaches provide high sensitivity and specificity, but the bulkiness of experimental instruments and complicated protocols hamper their use in resource-limited settings. In response, here we propose a smartphone-based device able to support laboratory-based optical techniques directly at the point of care. Specifically, we designed and fabricated a portable microplate reader that supports colorimetric, fluorescence, luminescence, and turbidity analyses. To demonstrate the potential of the device, we characterized its analytical performance by detecting a variety of relevant molecular targets (ranging from antibodies, toxins, drugs, and classic fluorophore dyes) and we showed how the estimated results are comparable to those obtained from a commercial microplate reader. Thanks to its low cost (<$300), portability (27 cm [length] × 18 cm [width] × 7 cm [height]), commercially available components, and open-source-based system, we believe it represents a valid approach to bring high-precision laboratory-based analysis at the point of care.

Bergua, J.f., Alvarez-Diduk, R., Idili, A., Parolo, C., Maymó, M., Hu, L., et al. (2022). Low-cost, user-friendly, all-integrated smartphone-based microplate reader for optical-based biological and chemical analyses. ANALYTICAL CHEMISTRY, 94(2), 1271-1285 [10.1021/acs.analchem.1c04491].

Low-cost, user-friendly, all-integrated smartphone-based microplate reader for optical-based biological and chemical analyses

A. Idili
Methodology
;
2022-01-03

Abstract

The quantitative detection of different molecular targets is of utmost importance for a variety of human activities, ranging from healthcare to environmental studies. Bioanalytical methods have been developed to solve this and to achieve the quantification of multiple targets from small volume samples. Generally, they can be divided into two different classes: point of care (PoC) and laboratory-based approaches. The former is rapid, low-cost, and user-friendly; however, the majority of the tests are semiquantitative, lacking in specificity and sensitivity. On the contrary, laboratory-based approaches provide high sensitivity and specificity, but the bulkiness of experimental instruments and complicated protocols hamper their use in resource-limited settings. In response, here we propose a smartphone-based device able to support laboratory-based optical techniques directly at the point of care. Specifically, we designed and fabricated a portable microplate reader that supports colorimetric, fluorescence, luminescence, and turbidity analyses. To demonstrate the potential of the device, we characterized its analytical performance by detecting a variety of relevant molecular targets (ranging from antibodies, toxins, drugs, and classic fluorophore dyes) and we showed how the estimated results are comparable to those obtained from a commercial microplate reader. Thanks to its low cost (<$300), portability (27 cm [length] × 18 cm [width] × 7 cm [height]), commercially available components, and open-source-based system, we believe it represents a valid approach to bring high-precision laboratory-based analysis at the point of care.
3-gen-2022
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
https://pubs.acs.org/doi/full/10.1021/acs.analchem.1c04491
Bergua, J.f., Alvarez-Diduk, R., Idili, A., Parolo, C., Maymó, M., Hu, L., et al. (2022). Low-cost, user-friendly, all-integrated smartphone-based microplate reader for optical-based biological and chemical analyses. ANALYTICAL CHEMISTRY, 94(2), 1271-1285 [10.1021/acs.analchem.1c04491].
Bergua, Jf; Alvarez-Diduk, R; Idili, A; Parolo, C; Maymó, M; Hu, L; Merkoci, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/312619
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