Objective of the present study was then to assess the antiviral activity of the protease inhibitor amprenavir in macrophages (M/M), and to compare it with its efficacy in peripheral blood lymphocytes (PBL). M/M were obtained from blood of sero-negative healthy donors and infected with M-tropic HIV-1 strain (HIV-1(Ba-L)). The stabilized infection was assessed by monitoring the HIV-1 p24 gag antigen production in the supernatants of M/M cultures. In the setting of acute infection (treatment before HIV-1 challenge), amprenavir showed substantial activity both in M/M and PBL at similar concentrations (EC50: 0.011 and 0.031 muM, respectively); complete inhibition of HIV-1 replication was achieved in both cell types at concentration of about 2 muM. In the setting of chronical infection (i.e. antiviral treatment several days after established infection), an antiviral effect of amprenavir was achieved in M/M, but at concentrations higher than those active in acutely infected M/M (EC50: 0.72 muM, EC90: 18.2 muM). The antiviral effect in chronically infected M/M was sustained for at least 2 weeks of continuous treatment. These findings suggest that amprenavir (at relatively high concentrations) has a clinically relevant antiviral effect in persistently infected reservoirs of HIV. (C) 2003 Elsevier B.V. All rights reserved.
Aquaro, S., Guenci, T., Di Santo, F., Francesconi, M., Calio, R., & Perno, C.F. (2004). Potent antiviral activity of amprenavir in primary macrophages infected by human immunodeficiency virus. ANTIVIRAL RESEARCH, 61(2), 133-137.
|Tipologia:||Articolo su rivista|
|Citazione:||Aquaro, S., Guenci, T., Di Santo, F., Francesconi, M., Calio, R., & Perno, C.F. (2004). Potent antiviral activity of amprenavir in primary macrophages infected by human immunodeficiency virus. ANTIVIRAL RESEARCH, 61(2), 133-137.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/07 - Microbiologia e Microbiologia Clinica|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.antiviral.2003.09.002|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2004|
|Titolo:||Potent antiviral activity of amprenavir in primary macrophages infected by human immunodeficiency virus|
|Autori:||Aquaro, S; Guenci, T; Di Santo, F; Francesconi, M; Calio, R; Perno, CF|
|Appare nelle tipologie:||01 - Articolo su rivista|