Influenza is a significant cause of morbidity and mortality worldwide. Here we show changes in the abundance and activation states of more than 50 immune cell subsets in 35 individuals over 11 time points during human A/California/2009 (H1N1) virus challenge monitored using mass cytometry along with other clinical assessments. Peak change in monocyte, B cell, and T cell subset frequencies coincided with peak virus shedding, followed by marked activation of T and NI< cells. Results led to the identification of C038 as a critical regulator of plasmacytoid dendritic cell function in response to influenza virus. Machine learning using study-derived clinical parameters and single-cell data effectively classified and predicted susceptibility to infection. The coordinated immune cell dynamics defined in this study provide a framework for identifying novel correlates of protection in the evaluation of future influenza therapeutics.

Rahil, Z., Leylek, R., Schürch, C.m., Chen, H., Bjornson-Hooper, Z., Christensen, S.r., et al. (2020). Landscape of coordinated immune responses to H1N1 challenge in humans. THE JOURNAL OF CLINICAL INVESTIGATION, 130(11), 5800-5816 [10.1172/JCI137265].

Landscape of coordinated immune responses to H1N1 challenge in humans

Chen, Han;Gherardini, Pier Federico;
2020-11-02

Abstract

Influenza is a significant cause of morbidity and mortality worldwide. Here we show changes in the abundance and activation states of more than 50 immune cell subsets in 35 individuals over 11 time points during human A/California/2009 (H1N1) virus challenge monitored using mass cytometry along with other clinical assessments. Peak change in monocyte, B cell, and T cell subset frequencies coincided with peak virus shedding, followed by marked activation of T and NI< cells. Results led to the identification of C038 as a critical regulator of plasmacytoid dendritic cell function in response to influenza virus. Machine learning using study-derived clinical parameters and single-cell data effectively classified and predicted susceptibility to infection. The coordinated immune cell dynamics defined in this study provide a framework for identifying novel correlates of protection in the evaluation of future influenza therapeutics.
2-nov-2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/11 - BIOLOGIA MOLECOLARE
English
Adaptive immunity
Immunology
Infectious disease
Influenza
Innate immunity
Rahil, Z., Leylek, R., Schürch, C.m., Chen, H., Bjornson-Hooper, Z., Christensen, S.r., et al. (2020). Landscape of coordinated immune responses to H1N1 challenge in humans. THE JOURNAL OF CLINICAL INVESTIGATION, 130(11), 5800-5816 [10.1172/JCI137265].
Rahil, Z; Leylek, R; Schürch, Cm; Chen, H; Bjornson-Hooper, Z; Christensen, Sr; Gherardini, Pf; Bhate, Ss; Spitzer, Mh; Fragiadakis, Gk; Mukherjee, N...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/312078
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