Here we show a general approach to achieve dissipative control over toehold-mediated strand-displacement, the most widely employed reaction in the field of DNA nanotechnology. The approach relies on rationally re-engineering the classic strand displacement reaction such that the high-energy invader strand (fuel) is converted into a low-energy waste product through an energy-dissipating reaction allowing the spontaneous return to the original state over time. We show that such dissipative control over the toehold-mediated strand displacement process is reversible (up to 10 cycles), highly controllable and enables unique temporal activation of DNA systems. We show here two possible applications of this strategy: the transient labelling of DNA structures and the additional temporal control of cascade reactions.

Del Grosso, E., Irmisch, P., Gentile, S., Prins, L.j., Seidel, R., Ricci, F. (2022). Dissipative control over the toehold-mediated DNA strand displacement reaction. ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, 61(23) [10.1002/anie.202201929].

Dissipative control over the toehold-mediated DNA strand displacement reaction

Del Grosso, Erica;Ricci, Francesco
2022-06-07

Abstract

Here we show a general approach to achieve dissipative control over toehold-mediated strand-displacement, the most widely employed reaction in the field of DNA nanotechnology. The approach relies on rationally re-engineering the classic strand displacement reaction such that the high-energy invader strand (fuel) is converted into a low-energy waste product through an energy-dissipating reaction allowing the spontaneous return to the original state over time. We show that such dissipative control over the toehold-mediated strand displacement process is reversible (up to 10 cycles), highly controllable and enables unique temporal activation of DNA systems. We show here two possible applications of this strategy: the transient labelling of DNA structures and the additional temporal control of cascade reactions.
7-giu-2022
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
Con Impact Factor ISI
DNA Nanotechnology
Dissipative Self-Assembly
Strand Displacement Reaction
Temporal Control
Del Grosso, E., Irmisch, P., Gentile, S., Prins, L.j., Seidel, R., Ricci, F. (2022). Dissipative control over the toehold-mediated DNA strand displacement reaction. ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, 61(23) [10.1002/anie.202201929].
Del Grosso, E; Irmisch, P; Gentile, S; Prins, Lj; Seidel, R; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/311669
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