Background: The objective of this study was to establish the age and sex-dependent reference intervals for coagulation assays evaluated in healthy children, ranging from 0 days to 16 years old. Methods: PT, aPTT, Fibrinogen (functional), Antithrombin activity, Protein C anticoagulant activity, Protein S free antigen, Thrombin time, D-Dimer, Von Willebrand Factor antigen, Lupus anticoagulant (screening), extrinsic and intrinsic pathway factors, and activated Protein C resistance were evaluated using STA-R Max(2). Results: A total of 1280 subjects (671 males and 609 females) were divided into five groups, according to their age: 0-15 days (n = 280, 174 M and 106 F), 15-30 days (n = 208, 101 M and 107 F), 1-6 months (n = 369, 178 M and 191 F), 6-12 months (n = 214, 110 M and 104 F), and 1-16 years (n = 209, 108 M and 101 F). The 95% reference intervals and the 90% CI were established using the Harrell-Davis bootstrap method and the bootstrap percentile method, respectively. Conclusions: The present study supports the concept that adult and pediatric subjects should be evaluated using different reference intervals, at least for some coagulation tests, to avoid misdiagnosis, which can potentially lead to serious consequences for patients and their families, and ultimately the healthcare system.

Di Felice, G., Vidali, M., Parisi, G., Pezzi, S., Di Pede, A., Deidda, G., et al. (2022). Reference Intervals for Coagulation Parameters in Developmental Hemostasis from Infancy to Adolescence. DIAGNOSTICS, 12(10), 2552 [10.3390/diagnostics12102552].

Reference Intervals for Coagulation Parameters in Developmental Hemostasis from Infancy to Adolescence

Porzio, Ottavia
2022-10-20

Abstract

Background: The objective of this study was to establish the age and sex-dependent reference intervals for coagulation assays evaluated in healthy children, ranging from 0 days to 16 years old. Methods: PT, aPTT, Fibrinogen (functional), Antithrombin activity, Protein C anticoagulant activity, Protein S free antigen, Thrombin time, D-Dimer, Von Willebrand Factor antigen, Lupus anticoagulant (screening), extrinsic and intrinsic pathway factors, and activated Protein C resistance were evaluated using STA-R Max(2). Results: A total of 1280 subjects (671 males and 609 females) were divided into five groups, according to their age: 0-15 days (n = 280, 174 M and 106 F), 15-30 days (n = 208, 101 M and 107 F), 1-6 months (n = 369, 178 M and 191 F), 6-12 months (n = 214, 110 M and 104 F), and 1-16 years (n = 209, 108 M and 101 F). The 95% reference intervals and the 90% CI were established using the Harrell-Davis bootstrap method and the bootstrap percentile method, respectively. Conclusions: The present study supports the concept that adult and pediatric subjects should be evaluated using different reference intervals, at least for some coagulation tests, to avoid misdiagnosis, which can potentially lead to serious consequences for patients and their families, and ultimately the healthcare system.
20-ott-2022
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA
English
coagulation
pediatrics
reference interval
Di Felice, G., Vidali, M., Parisi, G., Pezzi, S., Di Pede, A., Deidda, G., et al. (2022). Reference Intervals for Coagulation Parameters in Developmental Hemostasis from Infancy to Adolescence. DIAGNOSTICS, 12(10), 2552 [10.3390/diagnostics12102552].
Di Felice, G; Vidali, M; Parisi, G; Pezzi, S; Di Pede, A; Deidda, G; D'Agostini, M; Carletti, M; Ceccarelli, S; Porzio, O
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/310897
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