Dried, fixed uncoated neuron granule cells and their neurites have been imaged in air by an atomic force microscope (AFM) in the repulsive regime of contact mode. Neurons have also been observed after swelling with glutamate or decapping. Modifications induced by glutamate resulted in a drastic reduction in the cell body height (from 1.5 to 2.0 mu m for the untreated ones down to 0.7-1.0 mu m) and in an increased corrugation of the granule cell membrane. In the decapped neurons AFM revealed decreasing cell body height for an increasing amount of removed material during the decapping procedure. These results demonstrate that AFM is the ideal technique to observe cell corrugation and height modifications during cell treatments of neurobiological interest.

Cricenti, A., De Stasio, G., Generosi, R., Scarselli, M., Perfetti, P., Ciotti, M., et al. (1996). Native and modified uncoated neurons observed by atomic force microscopy. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY. A. VACUUM, SURFACES, AND FILMS, 14(3), 1741-1746 [10.1116/1.580330].

Native and modified uncoated neurons observed by atomic force microscopy

Scarselli, MA;
1996-03-25

Abstract

Dried, fixed uncoated neuron granule cells and their neurites have been imaged in air by an atomic force microscope (AFM) in the repulsive regime of contact mode. Neurons have also been observed after swelling with glutamate or decapping. Modifications induced by glutamate resulted in a drastic reduction in the cell body height (from 1.5 to 2.0 mu m for the untreated ones down to 0.7-1.0 mu m) and in an increased corrugation of the granule cell membrane. In the decapped neurons AFM revealed decreasing cell body height for an increasing amount of removed material during the decapping procedure. These results demonstrate that AFM is the ideal technique to observe cell corrugation and height modifications during cell treatments of neurobiological interest.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore FIS/03
English
atomic force microscopy
neurons
Cricenti, A., De Stasio, G., Generosi, R., Scarselli, M., Perfetti, P., Ciotti, M., et al. (1996). Native and modified uncoated neurons observed by atomic force microscopy. JOURNAL OF VACUUM SCIENCE & TECHNOLOGY. A. VACUUM, SURFACES, AND FILMS, 14(3), 1741-1746 [10.1116/1.580330].
Cricenti, A; De Stasio, G; Generosi, R; Scarselli, M; Perfetti, P; Ciotti, M; Mercanti, D; Casalbore, P; Margaritondo, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/309056
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