Water-membrane partition and aggregation behavior are fundamental aspects of the biological activity of antibiotic peptides, natural compounds causing the death of pathogenic organisms by perturbing the permeability of their membranes. A synthetic fluorescent analog of the natural lipopeptaibol trichogin GA IV was used to study its interaction with model membranes. Time-resolved fluorescence data show that in water, an equilibrium between monomers and small aggregates is present, the two species having different affinity for membranes. Therefore, association curves are strongly dependent on peptide concentration. A similar heterogeneity is present in the membrane phase, which strongly suggests the occurrence of a monomer-aggregate equilibrium in this case, too. The relative population of each species was determined and a strong correlation between the concentration of membrane-bound aggregates and membrane leakage was found, thereby suggesting that liposome perturbation is due to peptide aggregates only. Light-scattering measurements demonstrate that leakage is not due to liposome micellization. Moreover, experiments with markers of different sizes show that molecules with a diameter of -4 nm are released only to a minor extent. Overall, these results suggest that, within the concentration range explored, pore formation by peptide aggregates is the most likely mechanism of action for trichogin in membranes.

Stella, L., Mazzuca, C., Venanzi, M., Palleschi, A., Didone, M., Formaggio, F., et al. (2004). Aggregation and water-membrane partition as major determinants of the activity of the antibiotic peptide trichogin GA IV. BIOPHYSICAL JOURNAL, 86(2), 936-945 [10.1016/S0006-3495(04)74169-7].

Aggregation and water-membrane partition as major determinants of the activity of the antibiotic peptide trichogin GA IV

STELLA, LORENZO;MAZZUCA, CLAUDIA;VENANZI, MARIANO;PALLESCHI, ANTONIO;PISPISA, BASILIO
2004

Abstract

Water-membrane partition and aggregation behavior are fundamental aspects of the biological activity of antibiotic peptides, natural compounds causing the death of pathogenic organisms by perturbing the permeability of their membranes. A synthetic fluorescent analog of the natural lipopeptaibol trichogin GA IV was used to study its interaction with model membranes. Time-resolved fluorescence data show that in water, an equilibrium between monomers and small aggregates is present, the two species having different affinity for membranes. Therefore, association curves are strongly dependent on peptide concentration. A similar heterogeneity is present in the membrane phase, which strongly suggests the occurrence of a monomer-aggregate equilibrium in this case, too. The relative population of each species was determined and a strong correlation between the concentration of membrane-bound aggregates and membrane leakage was found, thereby suggesting that liposome perturbation is due to peptide aggregates only. Light-scattering measurements demonstrate that leakage is not due to liposome micellization. Moreover, experiments with markers of different sizes show that molecules with a diameter of -4 nm are released only to a minor extent. Overall, these results suggest that, within the concentration range explored, pore formation by peptide aggregates is the most likely mechanism of action for trichogin in membranes.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore CHIM/02 - Chimica Fisica
English
Con Impact Factor ISI
antibiotic agent; lipopeptide; monomer; peptide; trichogin; unclassified drug; antigen recognition; article; bilayer membrane; cell death; cell membrane permeability; hydrophobicity; light scattering; measurement; membrane binding; micellization; nonhuman; peptide analysis; protein aggregation; water transport; Anti-Bacterial Agents; Antimicrobial Cationic Peptides; Dimerization; Glycopeptides; Liposomes; Macromolecular Substances; Membrane Fluidity; Membrane Lipids; Membrane Proteins; Membranes, Artificial; Molecular Conformation; Peptides; Permeability; Porosity; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Surface Properties; Water
Stella, L., Mazzuca, C., Venanzi, M., Palleschi, A., Didone, M., Formaggio, F., et al. (2004). Aggregation and water-membrane partition as major determinants of the activity of the antibiotic peptide trichogin GA IV. BIOPHYSICAL JOURNAL, 86(2), 936-945 [10.1016/S0006-3495(04)74169-7].
Stella, L; Mazzuca, C; Venanzi, M; Palleschi, A; Didone, M; Formaggio, F; Toniolo, C; Pispisa, B
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons Creative Commons

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/30855
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 68
  • ???jsp.display-item.citation.isi??? 76
social impact