Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.
Caliskan, O.s., Massacci, G., Krahmer, N., Sacco, F. (2022). Phosphoproteomics and Organelle Proteomics in Pancreatic Islets, 2456, 123-140.
Phosphoproteomics and Organelle Proteomics in Pancreatic Islets
Sacco, Francesca
2022-01-01
Abstract
Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.File in questo prodotto:
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