Medulloblastoma (MB) is a childhood malignant brain tumour comprising four main subgroups characterized by different genetic alterations and rate of mortality. Among MB subgroups, patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Here we identify a previously unrecognized role of the pro-autophagy factor AMBRA1 in regulating MB. We demonstrate that AMBRA1 expression depends on c-MYC levels and correlates with Group 3 patient poor prognosis; also, knockdown of AMBRA1 reduces MB stem potential, growth and migration of MBGroup3 stem cells. At a molecular level, AMBRA1 mediates these effects by suppressing SOCS3, an inhibitor of STAT3 activation. Importantly, pharmacological inhibition of autophagy profoundly affects both stem and invasion potential of MBGroup3 stem cells, and a combined anti-autophagy and anti-STAT3 approach impacts the MBGroup3 outcome. Taken together, our data support the c-MYC/AMBRA1/STAT3 axis as a strong oncogenic signalling pathway with significance for both patient stratification strategies and targeted treatments of MBGroup3.

Nazio, F., Po, A., Abballe, L., Ballabio, C., Diomedi Camassei, F., Bordi, M., et al. (2021). Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling. ACTA NEUROPATHOLOGICA, 142(3), 537-564 [10.1007/s00401-021-02347-7].

Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling

Bordi, Matteo;Caruana, Ignazio;Campello, Silvia;Cecconi, Francesco
2021

Abstract

Medulloblastoma (MB) is a childhood malignant brain tumour comprising four main subgroups characterized by different genetic alterations and rate of mortality. Among MB subgroups, patients with enhanced levels of the c-MYC oncogene (MBGroup3) have the poorest prognosis. Here we identify a previously unrecognized role of the pro-autophagy factor AMBRA1 in regulating MB. We demonstrate that AMBRA1 expression depends on c-MYC levels and correlates with Group 3 patient poor prognosis; also, knockdown of AMBRA1 reduces MB stem potential, growth and migration of MBGroup3 stem cells. At a molecular level, AMBRA1 mediates these effects by suppressing SOCS3, an inhibitor of STAT3 activation. Importantly, pharmacological inhibition of autophagy profoundly affects both stem and invasion potential of MBGroup3 stem cells, and a combined anti-autophagy and anti-STAT3 approach impacts the MBGroup3 outcome. Taken together, our data support the c-MYC/AMBRA1/STAT3 axis as a strong oncogenic signalling pathway with significance for both patient stratification strategies and targeted treatments of MBGroup3.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/06
English
Autophagy
Brain tumours
Cancer stem cells
Therapy
Adaptor Proteins, Signal Transducing
Animals
Autophagy
Cell Line, Tumor
Cell Movement
Cerebellar Neoplasms
Child
Gene Knockdown Techniques
Humans
Medulloblastoma
Mice
Mice, Inbred C57BL
Neoplastic Stem Cells
Prognosis
Proto-Oncogene Proteins c-myc
STAT3 Transcription Factor
Signal Transduction
Suppressor of Cytokine Signaling 3 Protein
Nazio, F., Po, A., Abballe, L., Ballabio, C., Diomedi Camassei, F., Bordi, M., et al. (2021). Targeting cancer stem cells in medulloblastoma by inhibiting AMBRA1 dual function in autophagy and STAT3 signalling. ACTA NEUROPATHOLOGICA, 142(3), 537-564 [10.1007/s00401-021-02347-7].
Nazio, F; Po, A; Abballe, L; Ballabio, C; Diomedi Camassei, F; Bordi, M; Camera, A; Caruso, S; Caruana, I; Pezzullo, M; Ferraina, C; Milletti, G; Gianesello, M; Reddel, S; De Luca, Cd; Ceglie, D; Marinelli, S; Campello, S; Papaleo, E; Miele, E; Cacchione, A; Carai, A; Vinci, M; Velardi, E; De Angelis, B; Tiberi, L; Quintarelli, C; Mastronuzzi, A; Ferretti, E; Locatelli, F; Cecconi, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/303784
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