Point-of-care devices have attracted a huge interest by the scientific community because of the valuable potentiality for rapid diagnosis and precision medicine through cost-effective and easy-to-use devices for on-site measurement by unskilled personnel. Herein, we reported a smartphone-assisted electrochemical device consisted of a screen-printed electrode modified with carbon black nanomaterial and a commercially available smartphone potentiostat i.e. EmStat3 Blue, for sensitive detection of tyrosine. Once optimized the conditions, tyrosine was detected in standard solutions by square wave voltammetry, achieving a linear range comprised between 30 and 500 mu M, with a detection limit equal to 4.4 mu M. To detect tyrosine in serum, the interference of another amino acid i.e. tryptophan was hindered using a sample treatment with an extraction cartridge. The agreement of results analyzing serum samples with HPLC reference method and with the developed smart sensing system demonstrated the suitability of this smartphone-assisted sensing tool for cost-effective and rapid analyses of tyrosine in serum samples.

Fiore, L., De Lellis, B., Mazzaracchio, V., Suprun, E., Massoud, R., Goffredo, B.m., et al. (2022). Smartphone-assisted electrochemical sensor for reliable detection of tyrosine in serum. TALANTA, 237, 122869 [10.1016/j.talanta.2021.122869].

Smartphone-assisted electrochemical sensor for reliable detection of tyrosine in serum

Mazzaracchio V.
Conceptualization
;
Massoud R.
Validation
;
Moscone D.
Supervision
;
2022

Abstract

Point-of-care devices have attracted a huge interest by the scientific community because of the valuable potentiality for rapid diagnosis and precision medicine through cost-effective and easy-to-use devices for on-site measurement by unskilled personnel. Herein, we reported a smartphone-assisted electrochemical device consisted of a screen-printed electrode modified with carbon black nanomaterial and a commercially available smartphone potentiostat i.e. EmStat3 Blue, for sensitive detection of tyrosine. Once optimized the conditions, tyrosine was detected in standard solutions by square wave voltammetry, achieving a linear range comprised between 30 and 500 mu M, with a detection limit equal to 4.4 mu M. To detect tyrosine in serum, the interference of another amino acid i.e. tryptophan was hindered using a sample treatment with an extraction cartridge. The agreement of results analyzing serum samples with HPLC reference method and with the developed smart sensing system demonstrated the suitability of this smartphone-assisted sensing tool for cost-effective and rapid analyses of tyrosine in serum samples.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01
English
Con Impact Factor ISI
Square wave voltammetry
Extraction cartridge
Screen-printed electrode
Carbon black nanomaterial
Biological fluid
Tyrosine
Fiore, L., De Lellis, B., Mazzaracchio, V., Suprun, E., Massoud, R., Goffredo, B.m., et al. (2022). Smartphone-assisted electrochemical sensor for reliable detection of tyrosine in serum. TALANTA, 237, 122869 [10.1016/j.talanta.2021.122869].
Fiore, L; De Lellis, B; Mazzaracchio, V; Suprun, E; Massoud, R; Goffredo, Bm; Moscone, D; Arduini, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/303594
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