DNA-templated chemical reactions have found wide applications in drug discovery, programmed multistep synthesis, nucleic acid detection, and targeted drug delivery. The control of these reactions has, however, been limited to nucleic acid hybridization as a means to direct the proximity between reactants. In this work a system capable of translating protein-protein binding events into a DNA-templated reaction which leads to the covalent formation of a product is introduced. Protein-templated reactions by employing two DNA-antibody conjugates that are both able to recognize the same target protein and to colocalize a pair of reactant DNA strands able to undergo a click reaction are achieved. Two individual systems, each responsive to human serum albumin (HSA) and human IgG, are engineered and it is demonstrated that, while no reaction occurs in the absence of proteins, both protein-templated reactions can occur simultaneously in the same solution without any inter-system crosstalk.

Baranda Pellejero, L., Nijenhuis, M., Ricci, F., Gothelf, K.v. (2022). Protein-templated reactions using DNA-antibody conjugates. SMALL [10.1002/smll.202200971].

Protein-templated reactions using DNA-antibody conjugates

Ricci F.
Membro del Collaboration Group
;
2022-01-01

Abstract

DNA-templated chemical reactions have found wide applications in drug discovery, programmed multistep synthesis, nucleic acid detection, and targeted drug delivery. The control of these reactions has, however, been limited to nucleic acid hybridization as a means to direct the proximity between reactants. In this work a system capable of translating protein-protein binding events into a DNA-templated reaction which leads to the covalent formation of a product is introduced. Protein-templated reactions by employing two DNA-antibody conjugates that are both able to recognize the same target protein and to colocalize a pair of reactant DNA strands able to undergo a click reaction are achieved. Two individual systems, each responsive to human serum albumin (HSA) and human IgG, are engineered and it is demonstrated that, while no reaction occurs in the absence of proteins, both protein-templated reactions can occur simultaneously in the same solution without any inter-system crosstalk.
2022
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
bioconjugation
DNA templated reactions
nanotechnology
proteins
proximity effect
Baranda Pellejero, L., Nijenhuis, M., Ricci, F., Gothelf, K.v. (2022). Protein-templated reactions using DNA-antibody conjugates. SMALL [10.1002/smll.202200971].
Baranda Pellejero, L; Nijenhuis, Mad; Ricci, F; Gothelf, Kv
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/302295
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