We report here the rational design and optimization of an antibody responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the “translator,” that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control artificial protein-protein communication in artificial regulatory networks.

Ranallo, S., Sorrentino, D., Delibato, E., Ercolani, G., Plaxco, K.w., Ricci, F. (2022). Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device. ANGEWANDTE CHEMIE, 134(12) [10.1002/ange.202115680].

Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device

Ranallo, Simona;Sorrentino, Daniela;Ercolani, Gianfranco;Ricci, Francesco
2022-01-01

Abstract

We report here the rational design and optimization of an antibody responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the “translator,” that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control artificial protein-protein communication in artificial regulatory networks.
2022
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/01 - CHIMICA ANALITICA
English
DNA Nanotechnology; Aptamers; Synthetic biology; Supramolecular chemistry
This work received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement n. 843179 (“DNANANO-AB”, S.R.). The work was also supported by Associazione Italiana per la Ricerca sul Cancro, AIRC (project n. 21965 F.R.) and by the European Research Council, ERC (Consolidator Grant project n. 819160, F.R.)
Ranallo, S., Sorrentino, D., Delibato, E., Ercolani, G., Plaxco, K.w., Ricci, F. (2022). Non-natural protein-protein communication mediated by a DNA-based, antibody-responsive device. ANGEWANDTE CHEMIE, 134(12) [10.1002/ange.202115680].
Ranallo, S; Sorrentino, D; Delibato, E; Ercolani, G; Plaxco, Kw; Ricci, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/299511
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