High-frequency ultrasound treatment is found to be a one-pot green technique to produce peptide-based nanostructures by ultrasound assisted self-assembly of oligopeptides. [Arg-Phe]4 octapeptides, consisting of alternating arginine (Arg/R) and phenylalanine (Phe/F) sequences, were subjected to 430 kHz ultrasound in aqueous solution in the absence of any external agents, to form [RF]4 nanoparticles ([RF]4-NPs), ~220 nm in diameter. A comprehensive analysis of the obtained nanoparticles demonstrated that the aromatic moieties of the oligopeptides can undergo oxidative coupling to form multiple oligomeric species, which then self-assemble into well-defined fluorescent nanoparticles. [RF]4-NPs were functionalized with polyethylene glycol (PEGylated) to improve their colloidal stability. Unlike the parent peptide, the PEGylated [RF]4-NPs showed limited cytotoxicity towards MDA-MB-231 cells. Furthermore, the intracellular trafficking of PEGylated [RF]4-NPs was investigated after incubation with MDA-MB-231 cells to demonstrate their efficient endo-lysosomal escape. This work highlights that the combined use of ultrasonic technologies and peptides enables easy fabrication of nanoparticles, with potential application in drug delivery.

Baral, A., Bhangu, S.k., Cimino, R., Pelin, J., Alves, W.a., Chattopadhyay, S., et al. (2020). Sono-assembly of the [Arg-Phe]4 octapeptide into biofunctional nanoparticles. NANOMATERIALS, 10(9), 1-12 [10.3390/nano10091772].

Sono-assembly of the [Arg-Phe]4 octapeptide into biofunctional nanoparticles

Cavalieri F.
2020-09-08

Abstract

High-frequency ultrasound treatment is found to be a one-pot green technique to produce peptide-based nanostructures by ultrasound assisted self-assembly of oligopeptides. [Arg-Phe]4 octapeptides, consisting of alternating arginine (Arg/R) and phenylalanine (Phe/F) sequences, were subjected to 430 kHz ultrasound in aqueous solution in the absence of any external agents, to form [RF]4 nanoparticles ([RF]4-NPs), ~220 nm in diameter. A comprehensive analysis of the obtained nanoparticles demonstrated that the aromatic moieties of the oligopeptides can undergo oxidative coupling to form multiple oligomeric species, which then self-assemble into well-defined fluorescent nanoparticles. [RF]4-NPs were functionalized with polyethylene glycol (PEGylated) to improve their colloidal stability. Unlike the parent peptide, the PEGylated [RF]4-NPs showed limited cytotoxicity towards MDA-MB-231 cells. Furthermore, the intracellular trafficking of PEGylated [RF]4-NPs was investigated after incubation with MDA-MB-231 cells to demonstrate their efficient endo-lysosomal escape. This work highlights that the combined use of ultrasonic technologies and peptides enables easy fabrication of nanoparticles, with potential application in drug delivery.
8-set-2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore ING-IND/23 - CHIMICA FISICA APPLICATA
English
[Arg-Phe]4 octapeptides; acoustic cavitation; nanoparticles; self-assembly; sono-assembly
Baral, A., Bhangu, S.k., Cimino, R., Pelin, J., Alves, W.a., Chattopadhyay, S., et al. (2020). Sono-assembly of the [Arg-Phe]4 octapeptide into biofunctional nanoparticles. NANOMATERIALS, 10(9), 1-12 [10.3390/nano10091772].
Baral, A; Bhangu, Sk; Cimino, R; Pelin, Jnbd; Alves, Wa; Chattopadhyay, S; Ashokkumar, M; Cavalieri, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/296749
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