An enzyme catalase biosensor was fabricated to determine hydrogen peroxide. The enzyme was cross-linked with [ZnIIAlIII (OH)2]+ NO3– Layered Double Hydroxide (LDH), which was glued on a glassy carbon (GC) electrode. The amperometric response to hydrogen peroxide was studied. A limit of detection (LOD) of about 0.20 mM and a good lifetime as high as 75 days were observed. A sensor with the same configuration, without the immobilized catalase enzyme and with LDH glued on (GC), was also realized. This non-enzymatic sensor also showed catalytic activity towards H2O2, exhibiting an amperometric response obviously much lower than that of the enzyme biosensor, with a LOD of about 1.0 mM. However, also the lifetime of this catalytic sensor was longer than 2 months after preparation.

Tomassetti, M., Pezzilli, R., Prestopino, G., Di Natale, C., Medaglia, P.g. (2021). Fabrication and characterization of a Layered Double Hydroxide based catalase biosensor and a catalytic sensor for hydrogen peroxide determination. MICROCHEMICAL JOURNAL, 170 [10.1016/j.microc.2021.106700].

Fabrication and characterization of a Layered Double Hydroxide based catalase biosensor and a catalytic sensor for hydrogen peroxide determination

Di Natale C.;Medaglia P. G.
2021

Abstract

An enzyme catalase biosensor was fabricated to determine hydrogen peroxide. The enzyme was cross-linked with [ZnIIAlIII (OH)2]+ NO3– Layered Double Hydroxide (LDH), which was glued on a glassy carbon (GC) electrode. The amperometric response to hydrogen peroxide was studied. A limit of detection (LOD) of about 0.20 mM and a good lifetime as high as 75 days were observed. A sensor with the same configuration, without the immobilized catalase enzyme and with LDH glued on (GC), was also realized. This non-enzymatic sensor also showed catalytic activity towards H2O2, exhibiting an amperometric response obviously much lower than that of the enzyme biosensor, with a LOD of about 1.0 mM. However, also the lifetime of this catalytic sensor was longer than 2 months after preparation.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore ING-INF/01
English
Catalase biosensor
Catalytic LDH sensor
Hydrogen peroxide determination
LDH
Tomassetti, M., Pezzilli, R., Prestopino, G., Di Natale, C., Medaglia, P.g. (2021). Fabrication and characterization of a Layered Double Hydroxide based catalase biosensor and a catalytic sensor for hydrogen peroxide determination. MICROCHEMICAL JOURNAL, 170 [10.1016/j.microc.2021.106700].
Tomassetti, M; Pezzilli, R; Prestopino, G; Di Natale, C; Medaglia, Pg
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/296037
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