The embryonal rhabdomyosarcoma (eRMS) is a soft tissue sarcoma commonly affecting the head and neck, the extremities and the genitourinary tract. To contribute to revealing the cell types that may originate this tumor, we exploited mass cytometry, a single-cell technique that, by using heavy-metal-tagged antibodies, allows the accurate monitoring of the changes occurring in the mononuclear cell composition of skeletal muscle tissue during tumor development. To this end, we compared cell populations of healthy muscles with those from spatiotemporal-induced eRMS tumors in a mouse model (LSL-Kras(G12D/+);Tp53(Fl/Fl)) that can be used to develop rhabdomyosarcoma by means of infection with an adenovirus vector expressing Cre (Ad-Cre) recombinase. By monitoring different time points after tumor induction, we were able to analyze tumor progression and composition, identifying fibro/adipogenic progenitors (FAPs) as the cell type that, in this model system, had a pivotal role in tumor development. In vitro studies highlighted that both FAPs and satellite cells (SCs), upon infection with the Ad-Cre, acquired the potential to develop rhabdomyosarcomas when transplanted into immunocompromised mice. However, only infected FAPs had an antigen profile that was similar to embryonal rhabdomyosarcoma cells. Overall, our analysis supports the involvement of FAPs in eRMS development.

Petrilli, L.l., Riccio, F., Giuliani, G., Palma, A., Gargioli, C., Vumbaca, S., et al. (2021). Skeletal muscle subpopulation rearrangements upon rhabdomyosarcoma development through single-cell mass cytometry. JOURNAL OF CLINICAL MEDICINE, 10(4), 1-22 [10.3390/jcm10040823].

Skeletal muscle subpopulation rearrangements upon rhabdomyosarcoma development through single-cell mass cytometry

Gargioli C.;Sacco F.;Cesareni G.;Castagnoli L.;Fuoco C.
2021-01-01

Abstract

The embryonal rhabdomyosarcoma (eRMS) is a soft tissue sarcoma commonly affecting the head and neck, the extremities and the genitourinary tract. To contribute to revealing the cell types that may originate this tumor, we exploited mass cytometry, a single-cell technique that, by using heavy-metal-tagged antibodies, allows the accurate monitoring of the changes occurring in the mononuclear cell composition of skeletal muscle tissue during tumor development. To this end, we compared cell populations of healthy muscles with those from spatiotemporal-induced eRMS tumors in a mouse model (LSL-Kras(G12D/+);Tp53(Fl/Fl)) that can be used to develop rhabdomyosarcoma by means of infection with an adenovirus vector expressing Cre (Ad-Cre) recombinase. By monitoring different time points after tumor induction, we were able to analyze tumor progression and composition, identifying fibro/adipogenic progenitors (FAPs) as the cell type that, in this model system, had a pivotal role in tumor development. In vitro studies highlighted that both FAPs and satellite cells (SCs), upon infection with the Ad-Cre, acquired the potential to develop rhabdomyosarcomas when transplanted into immunocompromised mice. However, only infected FAPs had an antigen profile that was similar to embryonal rhabdomyosarcoma cells. Overall, our analysis supports the involvement of FAPs in eRMS development.
2021
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/13 - BIOLOGIA APPLICATA
English
embryonal rhabdomyosarcoma
skeletal muscle
muscle populations
single cell
CyTOF
mass cytometry
tumor heterogeneity
mesenchymal cells
stem cells
Petrilli, L.l., Riccio, F., Giuliani, G., Palma, A., Gargioli, C., Vumbaca, S., et al. (2021). Skeletal muscle subpopulation rearrangements upon rhabdomyosarcoma development through single-cell mass cytometry. JOURNAL OF CLINICAL MEDICINE, 10(4), 1-22 [10.3390/jcm10040823].
Petrilli, Ll; Riccio, F; Giuliani, G; Palma, A; Gargioli, C; Vumbaca, S; Faron, M; Palmieri, G; Pasquini, L; Sacco, F; Cesareni, G; Castagnoli, L; Fuo...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/295651
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