The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue loss often leads to a chronic pathologic condition known as volumetric muscle loss. Here, we propose a biofabrication approach to rapidly restore skeletal muscle mass, 3D histoarchitecture, and functionality. By recapitulating muscle anisotropic organization at the microscale level, we demonstrate to efficiently guide cell differentiation and myobundle formation both in vitro and in vivo. Of note, upon implantation, the biofabricated myo-substitutes support the formation of new blood vessels and neuromuscular junctions-pivotal aspects for cell survival and muscle contractile functionalities-together with an advanced muscle mass and force recovery. Altogether, these data represent a solid base for further testing the myo-substitutes in large animal size and a promising platform to be eventually translated into clinical scenarios.

Costantini, M., Testa, S., Fornetti, E., Fuoco, C., Sanchez Riera, C., Nie, M., et al. (2021). Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration. EMBO MOLECULAR MEDICINE, 13(3), e12778 [10.15252/emmm.202012778].

Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration

Testa S.;Fuoco C.;Rainer A.;Castagnoli L.;Cesareni G.;Cannata S.;Gargioli C.
2021

Abstract

The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue loss often leads to a chronic pathologic condition known as volumetric muscle loss. Here, we propose a biofabrication approach to rapidly restore skeletal muscle mass, 3D histoarchitecture, and functionality. By recapitulating muscle anisotropic organization at the microscale level, we demonstrate to efficiently guide cell differentiation and myobundle formation both in vitro and in vivo. Of note, upon implantation, the biofabricated myo-substitutes support the formation of new blood vessels and neuromuscular junctions-pivotal aspects for cell survival and muscle contractile functionalities-together with an advanced muscle mass and force recovery. Altogether, these data represent a solid base for further testing the myo-substitutes in large animal size and a promising platform to be eventually translated into clinical scenarios.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/13
eng
bioprinting
skeletal muscle
stem cells
tissue engineering
VML
Animals
Cell Differentiation
Humans
Mice
Muscle, Skeletal
Muscular Diseases
Tissue Engineering
Costantini, M., Testa, S., Fornetti, E., Fuoco, C., Sanchez Riera, C., Nie, M., et al. (2021). Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration. EMBO MOLECULAR MEDICINE, 13(3), e12778 [10.15252/emmm.202012778].
Costantini, M; Testa, S; Fornetti, E; Fuoco, C; Sanchez Riera, C; Nie, M; Bernardini, S; Rainer, A; Baldi, J; Zoccali, C; Biagini, R; Castagnoli, L; Vitiello, L; Blaauw, B; Seliktar, D; Swieszkowski, W; Garstecki, P; Takeuchi, S; Cesareni, G; Cannata, S; Gargioli, C
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/295649
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