AMP-activated protein kinase (AMPK) is currently the subject of intensive study and active discussions. AMPK performs its functions both at the cellular level, providing the switch between energy-consuming and energy-producing processes, and at the whole body level, particularly, regulating certain aspects of higher nervous activity and behavior. Control of such a 'main switch' compensates dysfunctions and associated diseases. In the present paper, we studied the binding of 3-benzylidene oxindoles to the kinase domain of the AMPK alpha-subunit, which is thought to prevent its interaction with the autoinhibitory domain and thus result in the AMPK activation. For this purpose, we developed the cellular test system based on the AMPKAR plasmid, which implements the FRET effect, synthesized a number of 3-benzylidene oxindole compounds and simulated their binding to various sites of the kinase domain. The most probable binding site for the studied compounds was established by the correlation of calculated and experimental data. The obtained results allow to analyze various classes of AMPK activators using virtual and high-content screening.

Novikova, D.s., Grigoreva, T.a., Ivanov, G.s., Melino, G., Barlev, N.a., Tribulovich, V.g. (2020). Activating Effect of 3-Benzylidene Oxindoles on {AMPK}: From Computer Simulation to High-Content Screening. CHEMBIOCHEM, 15(24), 2521-2529 [10.1002/cmdc.202000579].

Activating Effect of 3-Benzylidene Oxindoles on {AMPK}: From Computer Simulation to High-Content Screening

Gennaro Melino;
2020-01-01

Abstract

AMP-activated protein kinase (AMPK) is currently the subject of intensive study and active discussions. AMPK performs its functions both at the cellular level, providing the switch between energy-consuming and energy-producing processes, and at the whole body level, particularly, regulating certain aspects of higher nervous activity and behavior. Control of such a 'main switch' compensates dysfunctions and associated diseases. In the present paper, we studied the binding of 3-benzylidene oxindoles to the kinase domain of the AMPK alpha-subunit, which is thought to prevent its interaction with the autoinhibitory domain and thus result in the AMPK activation. For this purpose, we developed the cellular test system based on the AMPKAR plasmid, which implements the FRET effect, synthesized a number of 3-benzylidene oxindole compounds and simulated their binding to various sites of the kinase domain. The most probable binding site for the studied compounds was established by the correlation of calculated and experimental data. The obtained results allow to analyze various classes of AMPK activators using virtual and high-content screening.
2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/11 - BIOLOGIA MOLECOLARE
Settore BIO/10 - BIOCHIMICA
English
AMPK
FRET
high-content screening
molecular modeling
structure-activity relationships
Novikova, D.s., Grigoreva, T.a., Ivanov, G.s., Melino, G., Barlev, N.a., Tribulovich, V.g. (2020). Activating Effect of 3-Benzylidene Oxindoles on {AMPK}: From Computer Simulation to High-Content Screening. CHEMBIOCHEM, 15(24), 2521-2529 [10.1002/cmdc.202000579].
Novikova, Ds; Grigoreva, Ta; Ivanov, Gs; Melino, G; Barlev, Na; Tribulovich, Vg
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/294557
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 9
social impact