The analysis of a lymphoblastoid cell line (5106), derived from a rare individual of normal intelligence with an unmethylated full mutation of the FMR1 gene, allowed us to reconstruct the chain of molecular events leading to the FMR1 inactivation and to fragile X syndrome. We found that lack of DNA methylation of the entire promoter region, including the expanded CGG repeat, correlates with methylation of lysine 4 residue on the N-tail of histone H3 (H3-K4), as in normal controls. Normal levels of FMR1 mRNA were detected by real-time fluorescent RT-PCR (0.8-1.4 times compared with a control sample), but mRNA translation was less efficient (-40%), as judged by polysome profiling, resulting in reduced levels of FMRP protein (similar to30% of a normal control). These results underline once more that CGG repeat amplification per se does not prevent FMR1 transcription and FMRP production in the absence of DNA methylation. Surprisingly, we found by chromatin immunoprecipitation that cell line 5106 has deacetylated histones H3 and H4 as well as methylated lysine 9 on histone H3 (H3-K9), like fragile X cell lines, in both the promoter and exon 1. This indicates that these two epigenetic marks (i.e. histone deacetylation and H3-K9 methylation) can be established in the absence of DNA methylation and do not interfere with active gene transcription, contrary to expectation. Our results also suggest that the molecular pathways regulating DNA and H3-K4 methylation are independent from those regulating histone acetylation and H3-K9 methylation.

Pietrobono R., T.E. (2005). Molecular dissection of the events leading to inactivation of the FMR1 gene. HUMAN MOLECULAR GENETICS, 14(2), 267-277 [10.1093/hmg/ddi024].

Molecular dissection of the events leading to inactivation of the FMR1 gene

BAGNI, CLAUDIA;
2005

Abstract

The analysis of a lymphoblastoid cell line (5106), derived from a rare individual of normal intelligence with an unmethylated full mutation of the FMR1 gene, allowed us to reconstruct the chain of molecular events leading to the FMR1 inactivation and to fragile X syndrome. We found that lack of DNA methylation of the entire promoter region, including the expanded CGG repeat, correlates with methylation of lysine 4 residue on the N-tail of histone H3 (H3-K4), as in normal controls. Normal levels of FMR1 mRNA were detected by real-time fluorescent RT-PCR (0.8-1.4 times compared with a control sample), but mRNA translation was less efficient (-40%), as judged by polysome profiling, resulting in reduced levels of FMRP protein (similar to30% of a normal control). These results underline once more that CGG repeat amplification per se does not prevent FMR1 transcription and FMRP production in the absence of DNA methylation. Surprisingly, we found by chromatin immunoprecipitation that cell line 5106 has deacetylated histones H3 and H4 as well as methylated lysine 9 on histone H3 (H3-K9), like fragile X cell lines, in both the promoter and exon 1. This indicates that these two epigenetic marks (i.e. histone deacetylation and H3-K9 methylation) can be established in the absence of DNA methylation and do not interfere with active gene transcription, contrary to expectation. Our results also suggest that the molecular pathways regulating DNA and H3-K4 methylation are independent from those regulating histone acetylation and H3-K9 methylation.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
fragile X mental retardation protein; histone H3; histone H4; lysine; messenger RNA; amino terminal sequence; article; controlled study; correlation function; DNA methylation; epigenetics; exon; fragile X syndrome; gene amplification; gene inactivation; gene mutation; genetic transcription; human; human cell; immunoprecipitation; intelligence; lymphoblastoid cell line; male; nucleotide repeat; nucleotide sequence; pathogenesis; polysome; priority journal; promoter region; protein synthesis; reverse transcription polymerase chain reaction; RNA translation; Epigenesis, Genetic; Female; Fragile X Mental Retardation Protein; Gene Silencing; Humans; Male; Mutation; Nerve Tissue Proteins; RNA-Binding Proteins
Pietrobono R., T.E. (2005). Molecular dissection of the events leading to inactivation of the FMR1 gene. HUMAN MOLECULAR GENETICS, 14(2), 267-277 [10.1093/hmg/ddi024].
Pietrobono, R; Tabolacci, E; Zalfa, F; Zito, I; Terracciano, A; Moscato, U; Bagni, C; Oostra, B; Chiurazzi, P; Neri, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/29170
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