The leader sequence of Mycobacterium tuberculosis Cu,Zn superoxide dismutase (Cu,ZnSOD) contains a prokaryotic membrane lipoprotein attachment site. In the present study. we have found that the protein, which exhibits detectable SOD activity, is lipid-modified and associated with the bacterial membrane when expressed either in Al. tuberculosis or in Escherichia coli. These results provide the first demonstration of lipid modification of a Cu,ZnSOD. An analysis of the sodC genes present in available databases indicates that the same signal for lipid modification is also present in the sodC gene products from other mycobacteria and Gram-positive bacteria and, uniquely, in two distinct sodC gene products from the Gram-negative bacterium Salmonella typhimurium. Evidence is also provided for an up-regulation of M. tuberculosis sodC in response to phagocytosis by human macrophages, suggesting that Cu,ZnSOD is involved in the mechanisms that facilitate mycobacterial intracellular growth.

D'Orazio, M., Folcarelli, S., Mariani, F., Colizzi, V., Rotilio, G., Battistoni, A. (2001). Lipid modification of the Cu,Zn superoxide dismutase from Mycobacterium tuberculosis. BIOCHEMICAL JOURNAL, 359(1), 17-22 [10.1042/0264-6021:3590017].

Lipid modification of the Cu,Zn superoxide dismutase from Mycobacterium tuberculosis

D'Orazio M.;Colizzi V.;Rotilio G.;Battistoni A.
2001-01-01

Abstract

The leader sequence of Mycobacterium tuberculosis Cu,Zn superoxide dismutase (Cu,ZnSOD) contains a prokaryotic membrane lipoprotein attachment site. In the present study. we have found that the protein, which exhibits detectable SOD activity, is lipid-modified and associated with the bacterial membrane when expressed either in Al. tuberculosis or in Escherichia coli. These results provide the first demonstration of lipid modification of a Cu,ZnSOD. An analysis of the sodC genes present in available databases indicates that the same signal for lipid modification is also present in the sodC gene products from other mycobacteria and Gram-positive bacteria and, uniquely, in two distinct sodC gene products from the Gram-negative bacterium Salmonella typhimurium. Evidence is also provided for an up-regulation of M. tuberculosis sodC in response to phagocytosis by human macrophages, suggesting that Cu,ZnSOD is involved in the mechanisms that facilitate mycobacterial intracellular growth.
2001
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
sodC
anti-oxidant enzyme
virulence factor
lipoprotein
Salmonella
Cell Membrane
DNA Primers
Escherichia coli
Humans
Macrophages
Mycobacterium tuberculosis
Palmitic Acid
Peptide Fragments
Phagocytosis
Polymerase Chain Reaction
Superoxide Dismutase
Up-Regulation
Escherichia coli Proteins
D'Orazio, M., Folcarelli, S., Mariani, F., Colizzi, V., Rotilio, G., Battistoni, A. (2001). Lipid modification of the Cu,Zn superoxide dismutase from Mycobacterium tuberculosis. BIOCHEMICAL JOURNAL, 359(1), 17-22 [10.1042/0264-6021:3590017].
D'Orazio, M; Folcarelli, S; Mariani, F; Colizzi, V; Rotilio, G; Battistoni, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/291657
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