Eight Phaseolus vulgaris L. landraces cultivated on farm in marginal areas of Central Italy (Lazio region) were investigated in order to evaluate chemical composition of storage proteins and secondary metabolites fractions. The total protein content showed some differences among landraces; the maximum value was next to 30 g for 100 g of dry weight. The seed storage proteins were screened by polyacrylamide gel electrophoresis (SDS/PAGE): seven landraces exhibited phaseolin patterns type S, one landrace showed a phaseolin pattern type T. A morphological analysis of cotyledon parenchyma performed by scanning electron microscopy (SEM) revealed differences in size of starch granules. Moreover the polyphenolic composition was investigated using HPLC-APCI; from the methanol extracts a flavonoid, kaempferol, and a coumarin, 5,7-dimethoxycoumarin, were identified. To our knowledge, this is the first tithe that 5,7-dimethoxycoumarin has been reported in P. vulgaris seeds.

Perazzini, R., Leonardi, D., Ruggeri, S., Alesiani, D., D'Arcangelo, G., Canini, A. (2008). Characterization of Phaseolus vulgaris L. landraces cultivated in Central Italy. PLANT FOODS FOR HUMAN NUTRITION, 63(4), 211-218 [10.1007/s11130-008-0095-7].

Characterization of Phaseolus vulgaris L. landraces cultivated in Central Italy

CANINI, ANTONELLA
2008-01-01

Abstract

Eight Phaseolus vulgaris L. landraces cultivated on farm in marginal areas of Central Italy (Lazio region) were investigated in order to evaluate chemical composition of storage proteins and secondary metabolites fractions. The total protein content showed some differences among landraces; the maximum value was next to 30 g for 100 g of dry weight. The seed storage proteins were screened by polyacrylamide gel electrophoresis (SDS/PAGE): seven landraces exhibited phaseolin patterns type S, one landrace showed a phaseolin pattern type T. A morphological analysis of cotyledon parenchyma performed by scanning electron microscopy (SEM) revealed differences in size of starch granules. Moreover the polyphenolic composition was investigated using HPLC-APCI; from the methanol extracts a flavonoid, kaempferol, and a coumarin, 5,7-dimethoxycoumarin, were identified. To our knowledge, this is the first tithe that 5,7-dimethoxycoumarin has been reported in P. vulgaris seeds.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/01
English
Con Impact Factor ISI
Coumarins; Flavonoids; HPLC-APCI; Phaseolus vulgaris L.; Seed proteins; SEM
Perazzini, R., Leonardi, D., Ruggeri, S., Alesiani, D., D'Arcangelo, G., Canini, A. (2008). Characterization of Phaseolus vulgaris L. landraces cultivated in Central Italy. PLANT FOODS FOR HUMAN NUTRITION, 63(4), 211-218 [10.1007/s11130-008-0095-7].
Perazzini, R; Leonardi, D; Ruggeri, S; Alesiani, D; D'Arcangelo, G; Canini, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/29162
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