There is a growing interest in therapeutically targeting the inflammatory response that underlies age-related chronic diseases including obesity and type 2 diabetes. Through integrative small RNA sequencing, we show the presence of conserved plant miR159a and miR156c in dried nuts having high complementarity with the mammalian TNF receptor superfamily member 1a (Tnfrsf1a) transcript. We detected both miR159a and miR156c in exosome-like nut nanovesicles (NVs) and demonstrated that such NVs reduce Tnfrsf1a protein and dampen TNF-alpha signaling pathway in adipocytes. Synthetic single-stranded microRNAs (ss-miRs) modified with 2'-O-methyl group function as miR mimics. In plants, this modification naturally occurs on nearly all small RNAs. 2'-O-methylated ss-miR mimics for miR156c and miR159a decreased Tnfrsf1a protein and inflammatory markers in hypertrophic as well as TNF-alpha-treated adipocytes and macrophages. miR156c and miR159a mimics effectively suppress inflammation in mice, highlighting a potential role of plant miR-based, single-stranded oligonucleotides in treating inflammatory-associated metabolic diseases.

Aquilano, K., Ceci, V., Gismondi, A., De Stefano, S., Iacovelli, F., Faraonio, R., et al. (2019). Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts. COMMUNICATIONS BIOLOGY, 2(1), 317 [10.1038/s42003-019-0563-7].

Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts

Gismondi, Angelo;Iacovelli, Federico;Di Marco, Gabriele;Poerio, Noemi;Minutolo, Antonella;Fraziano, Maurizio;Bernardini, Roberta;Mattei, Maurizio;Falconi, Mattia;Montesano, Carla;Rufini, Stefano;Canini, Antonella;Lettieri-Barbato, Daniele
2019-01-01

Abstract

There is a growing interest in therapeutically targeting the inflammatory response that underlies age-related chronic diseases including obesity and type 2 diabetes. Through integrative small RNA sequencing, we show the presence of conserved plant miR159a and miR156c in dried nuts having high complementarity with the mammalian TNF receptor superfamily member 1a (Tnfrsf1a) transcript. We detected both miR159a and miR156c in exosome-like nut nanovesicles (NVs) and demonstrated that such NVs reduce Tnfrsf1a protein and dampen TNF-alpha signaling pathway in adipocytes. Synthetic single-stranded microRNAs (ss-miRs) modified with 2'-O-methyl group function as miR mimics. In plants, this modification naturally occurs on nearly all small RNAs. 2'-O-methylated ss-miR mimics for miR156c and miR159a decreased Tnfrsf1a protein and inflammatory markers in hypertrophic as well as TNF-alpha-treated adipocytes and macrophages. miR156c and miR159a mimics effectively suppress inflammation in mice, highlighting a potential role of plant miR-based, single-stranded oligonucleotides in treating inflammatory-associated metabolic diseases.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/09
Settore BIO/10
English
Disease model
Metabolism
Molecular medicine
3T3-L1 Cells
Adipocytes
Adipose Tissue
Animals
Cytokines
Female
Gene Expression Regulation, Plant
Glucose
HEK293 Cells
Humans
Hypertrophy
Inflammation
Insulin
Mice
Mice, Inbred C57BL
MicroRNAs
Nanoparticles
Nuts
RAW 264.7 Cells
RNA, Messenger
RNA, Plant
Receptors, Tumor Necrosis Factor
Desiccation
Aquilano, K., Ceci, V., Gismondi, A., De Stefano, S., Iacovelli, F., Faraonio, R., et al. (2019). Adipocyte metabolism is improved by TNF receptor-targeting small RNAs identified from dried nuts. COMMUNICATIONS BIOLOGY, 2(1), 317 [10.1038/s42003-019-0563-7].
Aquilano, K; Ceci, V; Gismondi, A; De Stefano, S; Iacovelli, F; Faraonio, R; Di Marco, G; Poerio, N; Minutolo, A; Minopoli, G; Marcone, A; Fraziano, M; Tortolici, F; Sennato, S; Casciardi, S; Potestà, M; Bernardini, R; Mattei, M; Falconi, M; Montesano, C; Rufini, S; Canini, A; Lettieri-Barbato, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/291178
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