: Response to ritonavir-boosted-protease inhibitors (PI/r)-based regimen is associated with some Gag mutations among HIV-1 B-clade. There is limited data on Gag mutations and their covariation with mutations in protease among HIV-1 non-B-clades at PI/r-based treatment failure. Thus, we characterized Gag mutations present in isolates from HIV-1 infected individuals treated with a PI/r-regimen (n = 143) and compared them with those obtained from individuals not treated with PI/r (ART-naïve [n = 101] or reverse transcriptase inhibitors (RTI) treated [n = 118]). The most frequent HIV-1 subtypes were CRF02_AG (54.69%), A (13.53%), D (6.35%) and G (4.69%). Eighteen Gag mutations showed a significantly higher prevalence in PI/r-treated isolates compared to ART-naïve (p < 0.05): Group 1 (prevalence < 1% in drug-naïve): L449F, D480N, L483Q, Y484P, T487V; group 2 (prevalence 1-5% in drug-naïve): S462L, I479G, I479K, D480E; group 3 (prevalence ≥ 5% in drug-naïve): P453L, E460A, R464G, S465F, V467E, Q474P, I479R, E482G, T487A. Five Gag mutations (L449F, P453L, D480E, S465F, Y484P) positively correlated (Phi ≥ 0.2, p < 0.05) with protease-resistance mutations. At PI/r-failure, no significant difference was observed between patients with and without these associated Gag mutations in term of viremia or CD4 count. This analysis suggests that some Gag mutations show an increased frequency in patients failing PIs among HIV-1 non-B clades.

Teto, G., Nka, A.d., Fokam, J., Bouba, Y., Takou, D., Fabeni, L., et al. (2022). Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients. SCIENTIFIC REPORTS, 12(1), 1374 [10.1038/s41598-022-05375-9].

Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients

Armenia, Daniele;Colizzi, Vittorio;Perno, Carlo-Federico;Ceccherini Silberstein, Francesca;Santoro, Maria;
2022-01-01

Abstract

: Response to ritonavir-boosted-protease inhibitors (PI/r)-based regimen is associated with some Gag mutations among HIV-1 B-clade. There is limited data on Gag mutations and their covariation with mutations in protease among HIV-1 non-B-clades at PI/r-based treatment failure. Thus, we characterized Gag mutations present in isolates from HIV-1 infected individuals treated with a PI/r-regimen (n = 143) and compared them with those obtained from individuals not treated with PI/r (ART-naïve [n = 101] or reverse transcriptase inhibitors (RTI) treated [n = 118]). The most frequent HIV-1 subtypes were CRF02_AG (54.69%), A (13.53%), D (6.35%) and G (4.69%). Eighteen Gag mutations showed a significantly higher prevalence in PI/r-treated isolates compared to ART-naïve (p < 0.05): Group 1 (prevalence < 1% in drug-naïve): L449F, D480N, L483Q, Y484P, T487V; group 2 (prevalence 1-5% in drug-naïve): S462L, I479G, I479K, D480E; group 3 (prevalence ≥ 5% in drug-naïve): P453L, E460A, R464G, S465F, V467E, Q474P, I479R, E482G, T487A. Five Gag mutations (L449F, P453L, D480E, S465F, Y484P) positively correlated (Phi ≥ 0.2, p < 0.05) with protease-resistance mutations. At PI/r-failure, no significant difference was observed between patients with and without these associated Gag mutations in term of viremia or CD4 count. This analysis suggests that some Gag mutations show an increased frequency in patients failing PIs among HIV-1 non-B clades.
gen-2022
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA
English
Adult
CD4 Lymphocyte Count
Cameroon
Drug Resistance, Viral
Female
Genotype
HIV Infections
HIV Protease
HIV Protease Inhibitors
HIV-1
Humans
Male
Middle Aged
Phylogeny
Prevalence
Reverse Transcriptase Inhibitors
Ritonavir
Treatment Failure
gag Gene Products, Human Immunodeficiency Virus
Mutation
Teto, G., Nka, A.d., Fokam, J., Bouba, Y., Takou, D., Fabeni, L., et al. (2022). Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients. SCIENTIFIC REPORTS, 12(1), 1374 [10.1038/s41598-022-05375-9].
Teto, G; Nka, Ad; Fokam, J; Bouba, Y; Takou, D; Fabeni, L; Carioti, L; Armenia, D; Semengue, Enj; Dambaya, B; Sosso, Sm; Colizzi, V; Perno, C; Ceccher...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/290037
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