Phosphatidylserine liposomes reduce inflammatory response, mycobacterial viability and HIV replication in coinfected human macrophages

: Chronic immune activation is the key pathogenetic event of Mycobacterium tuberculosis (MTB) and human immunodeficiency virus (HIV) coinfection. In the present study, we have assessed the therapeutic value of phosphatidylserine-liposome (PS-L) in an in vitro model of MTB/HIV coinfection. Our results show that PS-L reduce NF-κB activation and the downstream production of TNF-α, IL-1β and IL-6 in BCG-infected macrophages and of TNF-α and IL-1β in MTB-infected and in MTB/HIV-coinfected macrophages. Importantly, a significant reduction of intracellular MTB viability and HIV replication were also observed. These results support the further exploitation of PS-L as a host directed therapy for MTB/HIV coinfection.