External stimuli are potent tools that Nature uses to control protein function and activity. For instance, during viral entry and exit, pH variations are known to trigger large protein conformational changes. In Nature, also the electron transfer (ET) properties of ET proteins are influenced by pH-induced conformational changes. In this work, a pH-controlled, reversible 3(10)-helix to alpha-helix conversion (from acidic to highly basic pH values and vice versa) of a peptide supramolecular system built on a gold surface is described. The effect of pH on the ability of the peptide SAM to generate a photocurrent was investigated, with particular focus on the effect of the pH-induced conformational change on photocurrent efficiency. The films were characterized by electrochemical and spectroscopic techniques, and were found to be very stable over time, also in contact with a solution. They were also able to generate current under illumination, with an efficiency that is the highest recorded so far with biomolecular systems.

Kubitzky, S., Venanzi, M., Biondi, B., Lettieri, R., De Zotti, M., Gatto, E. (2021). A pH-induced reversible conformational switch able to control the photocurrent efficiency in a peptide supramolecular system. CHEMISTRY, 27(8), 2810-2817 [10.1002/chem.202004527].

A pH-induced reversible conformational switch able to control the photocurrent efficiency in a peptide supramolecular system

Venanzi M.
Supervision
;
Lettieri R.
Investigation
;
Gatto E.
2021-02-05

Abstract

External stimuli are potent tools that Nature uses to control protein function and activity. For instance, during viral entry and exit, pH variations are known to trigger large protein conformational changes. In Nature, also the electron transfer (ET) properties of ET proteins are influenced by pH-induced conformational changes. In this work, a pH-controlled, reversible 3(10)-helix to alpha-helix conversion (from acidic to highly basic pH values and vice versa) of a peptide supramolecular system built on a gold surface is described. The effect of pH on the ability of the peptide SAM to generate a photocurrent was investigated, with particular focus on the effect of the pH-induced conformational change on photocurrent efficiency. The films were characterized by electrochemical and spectroscopic techniques, and were found to be very stable over time, also in contact with a solution. They were also able to generate current under illumination, with an efficiency that is the highest recorded so far with biomolecular systems.
5-feb-2021
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore CHIM/02 - CHIMICA FISICA
English
conformational transition; electron transfer; peptides; photocurrent generation; supramolecular chemistry; Gold; Peptides; Protein Conformation; Hydrogen-Ion Concentration; Molecular Conformation
Kubitzky, S., Venanzi, M., Biondi, B., Lettieri, R., De Zotti, M., Gatto, E. (2021). A pH-induced reversible conformational switch able to control the photocurrent efficiency in a peptide supramolecular system. CHEMISTRY, 27(8), 2810-2817 [10.1002/chem.202004527].
Kubitzky, S; Venanzi, M; Biondi, B; Lettieri, R; De Zotti, M; Gatto, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/282487
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