The survival rate for head and neck cancer patients has not substantially changed in the last two decades. We previously showed that two rV-neuT intratumoral injections induced an efficient antitumor response and rejection of transplanted Neu (ratErbB2/neuoncogene-encoded protein)-overexpressing salivary gland tumor cells in BALB-neuT mice (BALB/c mice transgenic for the ratErbB2/neuoncogene). However, reiterated poxviral vaccinations increase neutralizing antibodies to viral proteins in humans that prevent immune response against the recombinant antigen expressed by the virus. Curcumin (CUR) is a polyphenol with antineoplastic and immunomodulatory properties. The aim of this study was to employ CUR administration to boost the anti-Neu immune response and anticancer activity induced by one rV-neuT intratumoral vaccination in BALB-neuT mice. Here, we demonstrated that the combined rV-neuT+CUR treatment was more effective at reducing tumor growth and increasing mouse survival, anti-Neu humoral response, and IFN-gamma/IL-2 T-cell release in vitro than the individual treatment. rV-neuT+CUR-treated mice showed an increased infiltration of CD4(+)/CD8(+)T lymphocytes within the tumor as compared to those that received the individual treatment. Overall, CUR enhanced the antitumoral effect and immune response to Neu induced by the rV-neuT vaccine in mice. Thus, the combined treatment might represent a successful strategy to target ErbB2/Neu-overexpressing tumors.

Focaccetti, C., Benvenuto, M., Ciuffa, S., Fazi, S., Scimeca, M., Nardi, A., et al. (2020). Curcumin enhances the antitumoral effect induced by the recombinant vaccinia neu vaccine (RV-NEUT) in mice with transplanted salivary gland carcinoma cells. NUTRIENTS, 12(5), 1417 [10.3390/nu12051417].

Curcumin enhances the antitumoral effect induced by the recombinant vaccinia neu vaccine (RV-NEUT) in mice with transplanted salivary gland carcinoma cells

Focaccetti C.;Benvenuto M.;Scimeca M.;Nardi A.;Bonanno E.;Modesti A.;Bei R.
2020-01-01

Abstract

The survival rate for head and neck cancer patients has not substantially changed in the last two decades. We previously showed that two rV-neuT intratumoral injections induced an efficient antitumor response and rejection of transplanted Neu (ratErbB2/neuoncogene-encoded protein)-overexpressing salivary gland tumor cells in BALB-neuT mice (BALB/c mice transgenic for the ratErbB2/neuoncogene). However, reiterated poxviral vaccinations increase neutralizing antibodies to viral proteins in humans that prevent immune response against the recombinant antigen expressed by the virus. Curcumin (CUR) is a polyphenol with antineoplastic and immunomodulatory properties. The aim of this study was to employ CUR administration to boost the anti-Neu immune response and anticancer activity induced by one rV-neuT intratumoral vaccination in BALB-neuT mice. Here, we demonstrated that the combined rV-neuT+CUR treatment was more effective at reducing tumor growth and increasing mouse survival, anti-Neu humoral response, and IFN-gamma/IL-2 T-cell release in vitro than the individual treatment. rV-neuT+CUR-treated mice showed an increased infiltration of CD4(+)/CD8(+)T lymphocytes within the tumor as compared to those that received the individual treatment. Overall, CUR enhanced the antitumoral effect and immune response to Neu induced by the rV-neuT vaccine in mice. Thus, the combined treatment might represent a successful strategy to target ErbB2/Neu-overexpressing tumors.
2020
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/04 - PATOLOGIA GENERALE
Settore MED/08 - ANATOMIA PATOLOGICA
English
ErbB2/Neu
cancer
curcumin
head and neck
immune response
tumor infiltrating leukocytes
vaccine
Animals
Antibody-Dependent Cell Cytotoxicity
Antineoplastic Agents
Cancer Vaccines
Carcinoma
Curcumin
Disease Models, Animal
Genes, erbB-2
Mice
Mice, Inbred BALB C
Recombination, Genetic
Salivary Gland Neoplasms
Salivary Glands
Vaccinia virus
Focaccetti, C., Benvenuto, M., Ciuffa, S., Fazi, S., Scimeca, M., Nardi, A., et al. (2020). Curcumin enhances the antitumoral effect induced by the recombinant vaccinia neu vaccine (RV-NEUT) in mice with transplanted salivary gland carcinoma cells. NUTRIENTS, 12(5), 1417 [10.3390/nu12051417].
Focaccetti, C; Benvenuto, M; Ciuffa, S; Fazi, S; Scimeca, M; Nardi, A; Miele, Mt; Battisti, A; Bonanno, E; Modesti, A; Masuelli, L; Bei, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/280475
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