Transient modification of the environment involves the expression of specific genes and degradation of mRNAs and proteins. How these events are linked is poorly understood. CCR4-NOT is an evolutionary conserved complex involved in transcription initiation and mRNA degradation. In this paper, we report that the yeast Not4 localizes in cytoplasmic foci after cellular stress. We focused our attention on the functional characterization of the C-terminus of the Not4 protein. Molecular dissection of this region indicates that the removal of the last 120 amino acids, does not affect protein localization and function, in that the protein is still able to suppress the thermosensitivity observed in the not4Δ mutant. In addition, such shortened form of Not4, as well its absence, increases the transcription of stress-responsive genes conferring to the cell high resistance to the oxidative stress. On the contrary, the last C-terminal 211 amino acids are required for proper Not4 localization at cytoplasmic foci after stress. This truncated version of Not4 fails to increase the transcription of the stress genes, is more stable and seems to be toxic to cells undergoing oxidative stress.

Palermo, V., Stirpe, M., Bianchi, M.m., Rinaldi, T., Cirigliano, A., Ragnini, A., et al. (2021). The C-terminal region of yeast ubiquitin-protein ligase Not4 mediates its cellular localization and stress response. FEMS MICROBIOLOGY LETTERS, 368(15) [10.1093/femsle/fnab097].

The C-terminal region of yeast ubiquitin-protein ligase Not4 mediates its cellular localization and stress response

Ragnini, Antonella
Investigation
;
2021-08-17

Abstract

Transient modification of the environment involves the expression of specific genes and degradation of mRNAs and proteins. How these events are linked is poorly understood. CCR4-NOT is an evolutionary conserved complex involved in transcription initiation and mRNA degradation. In this paper, we report that the yeast Not4 localizes in cytoplasmic foci after cellular stress. We focused our attention on the functional characterization of the C-terminus of the Not4 protein. Molecular dissection of this region indicates that the removal of the last 120 amino acids, does not affect protein localization and function, in that the protein is still able to suppress the thermosensitivity observed in the not4Δ mutant. In addition, such shortened form of Not4, as well its absence, increases the transcription of stress-responsive genes conferring to the cell high resistance to the oxidative stress. On the contrary, the last C-terminal 211 amino acids are required for proper Not4 localization at cytoplasmic foci after stress. This truncated version of Not4 fails to increase the transcription of the stress genes, is more stable and seems to be toxic to cells undergoing oxidative stress.
17-ago-2021
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/05 - ZOOLOGIA
English
Con Impact Factor ISI
E3 ubiquitin ligase
gene expression
protein aggregation
stress response
yeast
https://academic.oup.com/femsle/article/368/15/fnab097/6335481
Palermo, V., Stirpe, M., Bianchi, M.m., Rinaldi, T., Cirigliano, A., Ragnini, A., et al. (2021). The C-terminal region of yeast ubiquitin-protein ligase Not4 mediates its cellular localization and stress response. FEMS MICROBIOLOGY LETTERS, 368(15) [10.1093/femsle/fnab097].
Palermo, V; Stirpe, M; Bianchi, Mm; Rinaldi, T; Cirigliano, A; Ragnini, A; Falcone, C; Mazzoni, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/278116
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